Qhov sib txawv tseem ceeb ntawm CFSE thiab LFSE yog tias lo lus CFSE sawv cev rau cov tshuaj lom neeg, thaum lo lus LFSE sawv cev rau kev xav hauv chemistry.
Lub sij hawm CFSE sawv cev rau cov tshuaj lom neeg carboxyfluorescein succinimidyl ester. Nws yog ib qho xim uas peb feem ntau siv rau staining hlwb rau fluorescence. Lub sij hawm LFSE, ntawm qhov tod tes, stands rau ligand teb stabilization zog, uas yog ib lo lus nyob rau hauv Ligand teb txoj kev xav.
CFSE yog dab tsi?
Lub sij hawm CFSE sawv cev rau cov tshuaj lom neeg carboxyfluorescein succinimidyl ester. Nws muaj txiaj ntsig zoo li fluorescent cell staining dye. Cov tshuaj no yog cell-permeable thiab covalently khub niam txiv nrog intracellular molecules ntawm succinimidyl pawg ntawm CFSE. Qhov tseem ceeb, qhov no tshwm sim nrog intracellular lysine residues thiab lwm qhov chaw amine. Vim qhov kev sib txuas ntawm cov tshuaj tiv thaiv no ntawm CFSE molecules thiab intracellular molecules, cov fluorescent dye no khaws cia hauv cov hlwb rau lub sijhawm ntev heev.
Cov tshuaj formula ntawm CFSE molecule yog C25H15NO9. Qhov hnyav ntawm molar yog 473 g / mol. Feem ntau, CFSE tau tsis meej pem nrog CFDA-SE (carboxyfluorescein diacetate succinimidyl ester) vim lawv qhov zoo sib xws. Tab sis lawv tsis yog tib lub molecule, thiab CFSE yog cov cell-permeable piv rau CFDA-SE. Tsis tas li ntawd, qhov thib ob compound no tsis yog fluorescent.
Keeb kwm, CFSE compound tau tsim los ua cov xim fluorescent uas tuaj yeem ruaj khov daim ntawv lo lymphocytes thiab taug qab lawv txoj kev tsiv teb tsaws los ntawm cov ntshav hauv cov tsiaj ntev. Tom qab ntawd, nws tau nthuav tawm tias cov xim tuaj yeem siv los saib xyuas lymphocyte proliferation. Txawm li cas los xij, yog tias peb siv lub siab ntau hauv cov hlwb, nws muaj tshuaj lom rau tsiaj.
LFSE yog dab tsi?
Lo lus LFSE sawv cev rau ligand field stabilization zog uas yog ib lo lus nyob rau hauv Ligand teb txoj kev xav. Nws piav qhia txog kev sib raug zoo, kev npaj orbital thiab lwm yam ntxwv ntawm kev sib koom ua ke. Qhov kev xav no sawv cev rau ib qho kev siv ntawm molecular orbital txoj kev xav rau kev hloov cov hlau complexes. Feem ntau, cov hlau hloov pauv muaj cuaj valence atomic orbitals. Muaj tsib lub orbitals, ib lub orbital thiab peb lub orbitals uas tuaj yeem coj los ua valence shells. Cov orbitals no muaj lub zog tsim nyog los tsim cov tshuaj lom neeg nrog lub ligand. Lub zog no yog hu ua ligand field stabilization zog. Ntxiv mus, lub tswv yim tshuaj no feem ntau yog siv raws li lub geometry ntawm lub complex. Feem ntau, nws piav qhia txog cov octahedral complexes uas muaj rau ligands ib molecule.
Qhov txawv ntawm CFSE thiab LFSE yog dab tsi?
Lub sij hawm CFSE sawv cev rau cov tshuaj sib xyaw carboxyfluorescein succinimidyl ester thaum lub sij hawm LFSE sawv cev rau ligand teb stabilization zog uas yog ib lo lus nyob rau hauv Ligand teb txoj kev xav. Yog li, qhov sib txawv tseem ceeb ntawm CFSE thiab LFSE yog tias lo lus CFSE sawv cev rau cov tshuaj lom neeg, thaum lub sij hawm LFSE sawv cev rau kev xav hauv chemistry. Yuav kom paub meej ntxiv, CFSE yog fluorescent dye thaum LFSE yog lub zog yuav tsum tau ua kom ruaj khov rau kev sib koom tes.
Cov infographic hauv qab no qhia txog qhov sib txawv ntawm CFSE thiab LFSE hauv daim ntawv teev npe.
Summary – CFSE vs LFSE
Lub sij hawm CFSE sawv cev rau cov tshuaj sib xyaw carboxyfluorescein succinimidyl ester thaum lub sij hawm LFSE sawv cev rau ligand teb stabilization zog uas yog ib lo lus nyob rau hauv Ligand teb txoj kev xav. Yog li ntawd, qhov sib txawv tseem ceeb ntawm CFSE thiab LFSE yog tias lo lus CFSE sawv cev rau cov tshuaj lom neeg, thaum lub sij hawm LFSE sawv cev rau txoj kev xav hauv chemistry.
