Qhov sib txawv tseem ceeb ntawm cloning vector thiab kev qhia vector yog tias cloning vector nqa ib qho txawv teb chaws DNA tawg mus rau hauv lub xov tooj ntawm tes thaum qhia vector tswj kev qhia cov noob rau hauv cov protein.
Vector yog ib lo lus tseem ceeb hauv molecular biology. Hauv recombinant DNA thev naus laus zis, lub luag haujlwm tseem ceeb ntawm lub vector yog muab ib hom kev thauj mus rau DNA feem uas muaj txiaj ntsig kom tau muab tso rau hauv lub xov tooj ntawm tes. Tsis tas li ntawd, nws yog DNA molecule siv los nqa cov DNA txawv teb chaws cov khoom tsim tawm mus rau hauv lub xov tooj ntawm tes kom nthuav tawm lossis rov ua dua. Feem ntau siv vectors yog plasmids, kab mob vectors, cosmids thiab cov chromosomes dag. Cloning vector thiab kev qhia vector yog ob hom vectors cais raws li lawv daim ntawv thov.
Dab tsi yog Cloning Vector?
Ib cloning vector yog ib feem ntawm DNA uas tuaj yeem siv los ntxig cov DNA txawv teb chaws molecule thiab muaj peev xwm muab tso rau hauv ib lub party rau lub hom phiaj cloning. Qhov zoo tshaj plaws yam ntxwv ntawm cloning vector yog yooj yim ntxig / tshem tawm ntawm DNA tawg los ntawm kev txwv enzyme kho thiab ligating enzyme kho. Hauv qhov no, nquag siv cloning vectors yog cov genetically engineered plasmids.
Daim duab 01: Cloning Vector
Ib cloning vector yuav tsum muaj ntau qhov chaw cloning, xaiv cov noob caj noob ces thiab cov neeg sau xov xwm. Lub hom phiaj ntawm qhov chaw cloning yog los muab qhov chaw rau cloning tshwm sim. Ib qho kev xaiv noob caj noob ces pab txheeb xyuas cov kev sib txuas ua tiav tom qab cloning thiab cov neeg sau xov xwm gene tso cai rau kev tshuaj xyuas thiab txheeb xyuas qhov tseeb ntawm cov recombinant tom qab cloning. Lub cloning vector tsis tas yuav pab qhia cov protein uas txawv teb chaws DNA encodes. Yog li, tib lub hom phiaj ntawm cloning vector yog nqa cov DNA txawv teb chaws rau tus tswv.
Dab tsi yog kev nthuav qhia vector?
Expression vector, tseem hu ua kev tsim qauv, yog ib hom vector siv rau kev nthuav qhia cov proteins hauv lub xov tooj ntawm tes. Zoo li txhua tus vector, qhov no yuav tsum muaj cov ntsiab lus tseem ceeb ntawm ntau qhov chaw cloning, tus cim gene thiab tus neeg sau xov xwm gene. Lub vector qhia txog cov noob tshiab rau hauv tus tswv tsev thiab siv tus tswv tsev cov protein synthesis mechanism, nws tso cai rau cov noob tau nthuav tawm rau hauv tus tswv tsev. Ntxiv mus, nws qhov kev tsom xam thawj zaug yog ua kom ruaj khov m-RNA thiab yog li ua cov proteins. Ib qho piv txwv zoo yog kev lag luam tsim cov tshuaj insulin. Cov tshuaj insulin yog nkag mus rau cov kab mob plasmid thiab muab tso rau hauv E.coli kab mob hauv lub cev, tso cai rau cov plasmids sib faib thiab tso cai rau E. coli loj hlob, zais cov tshuaj insulin uas tuaj yeem sau tau thiab siv.
Daim duab 02: Kev nthuav qhia Vector
Tsis tas li ntawd, ib qho kev qhia vector yuav tsum muaj thaj chaw txhawb nqa muaj zog, kev pib txhais lus kom raug thiab qhov tseeb terminator codon thiab ib ntus. Kev nthuav qhia vectors muaj ntau daim ntawv thov hauv kev tsim cov peptides thiab cov proteins rau kev lag luam tshuaj xws li tsim cov tshuaj insulin, kev loj hlob hormone, tshuaj tua kab mob, tshuaj tiv thaiv, tshuaj tiv thaiv. Ntxiv mus, qhia vectors pab nyob rau hauv enzyme tsim nyob rau hauv cov zaub mov thiab tsoos tsho industries. Tsis tas li ntawd, kev qhia vectors yog qhov tseem ceeb hauv kev tsim cov nroj tsuag transgenic xws li cov nplej kub, cov nroj tsuag tiv taus kab, cov nroj tsuag tshuaj tua kab mob, thiab lwm yam.
Dab tsi yog qhov zoo sib xws ntawm Cloning Vector thiab Expression Vector?
- Cloning vector thiab kev qhia vector yog ob hom vectors peb siv nyob rau hauv recombinant DNA technology thiab genetic engineering.
- Ob leeg muaj tus cim cim thiab tus neeg sau xov xwm gene.
- Ntxiv mus, lawv muaj ntau qhov chaw cloning.
- Tsis tas li, lawv muaj keeb kwm ntawm kev rov ua dua, thiab muaj peev xwm ua tus kheej rov ua dua.
Qhov txawv ntawm Cloning Vector thiab Expression Vector yog dab tsi?
Ib cloning vector yog ib qho me me DNA molecule uas nqa cov DNA txawv teb chaws mus rau hauv lub xov tooj ntawm tes thaum qhia vector yog ib hom vector uas pab txhawb kev taw qhia, qhia cov noob thiab tsim cov proteins. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cloning vector thiab kev qhia vector. Tsis tas li ntawd, lwm qhov sib txawv tseem ceeb ntawm cloning vector thiab kev qhia vector yog tias cloning vector qhia txog DNA txawv teb chaws rau hauv ib tus tswv tsev thaum qhia vectors qhia cov noob qhia los ntawm kev tsim cov protein cuam tshuam.
Tsis tas li ntawd, cloning vector muaj keeb kwm ntawm kev rov ua dua, txwv qhov chaw, thiab tus cim xaiv tau. Thaum lub sij hawm, qhov kev qhia vector muaj cov kev txhim kho, thaj chaw txhawb nqa, kev txiav tawm codon, kev hloov pauv pib ua ntu zus, keeb kwm ntawm kev rov ua dua, qhov chaw txwv, thiab cov cim xaiv tau. Yog li, qhov no kuj yog qhov sib txawv ntawm cloning vector thiab kev qhia vector. Tsis tas li ntawd, plasmids, bacteriophages, cov kab mob ua kom zoo nkauj chromosomes, cosmids, mammalian dag chromosomes, poov xab dag chromosome, thiab lwm yam, yog cov piv txwv ntawm cloning vectors. Meanwhile, qhia vectors feem ntau yog plasmids.
Summary – Cloning Vector vs Expression Vector
Raws li lawv txoj haujlwm hauv molecular biology, muaj ob hom vectors li cloning vector thiab qhia vector. Ib tug cloning vector yog ib tug me me DNA molecule uas xa txawv teb chaws DNA mus rau hauv lub host cell. Muaj ntau hom cloning vectors xws li plasmids, bacteriophages, cov kab mob sib xyaw ua ke, cosmids, thiab mammalian artificial chromosomes. Hauv qhov sib piv, ib qho kev qhia vector yog plasmid uas qhia txog cov noob ntawm kev txaus siab rau hauv lub xov tooj ntawm tes thiab txhawb nqa cov noob qhia kom tau txais cov khoom lag luam protein. Kev qhia vectors yog plasmids. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm cloning vector thiab qhia vector.
