Qhov sib txawv tseem ceeb ntawm tus nqi zonal thiab isopycnic centrifugation yog tias tus nqi zonal centrifugation yog qhov tseem ceeb hauv kev sib cais cov khoom sib txawv tab sis tsis nyob hauv lawv qhov ntom, qhov isopycnic centrifugation yog qhov tseem ceeb hauv kev sib cais cov khoom sib txawv ntawm qhov ntom tab sis tsis nyob hauv lawv. size.
Centrifugation yog cov txheej txheem kev tshuaj xyuas peb tuaj yeem siv los cais cov khoom hauv cov tshuaj raws li qhov loj, zoo, qhov ntom, viscosity, thiab lwm yam ntawm qhov nruab nrab. Ntawm no, peb yuav tsum muab cov tshuaj uas muaj cov khoom tshem tawm hauv lub raj centrifugal thiab tom qab ntawd ntxig lub raj rau hauv rotor txhawm rau tig nws ntawm qhov ceev ceev kom cov khoom sib cais ntawm cov tshuaj.
Tus nqi Zonal centrifugation yog dab tsi?
Rate zonal centrifugation yog cov txheej txheem tshuaj ntsuam peb tuaj yeem siv los cais cov khoom ntawm cov tshuaj nyob ntawm qhov loj me, tab sis tsis nyob ntawm qhov ceev. Hauv lwm lo lus, cov txheej txheem no cais cov khoom los ntawm kev daws raws li qhov loj me, tab sis nws tsis xav txog qhov sib txawv ntawm qhov ntom ntawm cov khoom. Yog li ntawd, cov txheej txheem no muaj txiaj ntsig zoo hauv kev sib cais cellular organelles xws li endosomes. Ntxiv mus, peb tuaj yeem siv nws los cais cov proteins ib yam nkaus.
Daim duab 01: Lub Rooj Sab saum toj Centrifuge
Tsis tas li ntawd, qhov centrifugation no yog nyob ntawm lub sijhawm. Qhov no txhais tau hais tias txoj hauj lwm ntawm tus qauv cuam tshuam rau lub sij hawm ntawm sedimentation. Cov gradient peb tau los ntawm txoj kev no yog tiaj tus, thiab qhov siab tshaj plaws ntawm qhov gradient no yeej tsis tshaj qhov ceev ntawm qhov siab tshaj plaws ntawm cov khoom pellet.
Isopycnic centrifugation yog dab tsi?
Isopycnic centrifugation yog cov txheej txheem tshuaj ntsuam peb tuaj yeem siv los cais cov khoom ntawm cov tshuaj nyob ntawm qhov ntom, tab sis tsis yog qhov loj. Qhov ntawd txhais tau tias; peb tuaj yeem cais cov khoom hauv cov tshuaj yog tias lawv sib txawv ntawm ib leeg hauv lawv qhov ntom. Yog tias lawv txawv ntawm qhov loj me tsis yog qhov ntom, ces cov txheej txheem no tsis haum rau qhov kev sib cais. Qhov loj ntawm cov khoom cuam tshuam tsuas yog tus nqi ntawm kev tsiv teb tsaws ntawm cov khoom. Yog li ntawd, cov txheej txheem no yog ywj siab ntawm lub sijhawm.
Daim duab 02: Qhov ntom ntom ntom muab los ntawm Centrifugation
Qhov tseem ceeb tshaj, peb tuaj yeem siv cov txheej txheem no los cais cov nucleic acids hauv qhov gradient. Lub gradient muab los ntawm isopycnic centrifugation yog ntxhab. Ntxiv mus, nws tsis tsim ib lub pellet vim hais tias kev sib cais yog raws li buoyant densities ntawm particles.
Qhov txawv ntawm Tus Nqi Zonal thiab Isopycnic Centrifugation yog dab tsi?
Qhov sib txawv tseem ceeb ntawm tus nqi zonal thiab isopycnic centrifugation yog tias tus nqi zonal centrifugation yog qhov tseem ceeb hauv kev sib cais cov khoom sib txawv ntawm qhov loj tab sis tsis nyob hauv lawv qhov ntom, qhov isopycnic centrifugation tseem ceeb hauv kev sib cais cov khoom sib txawv ntawm qhov ntom tab sis tsis yog. hauv lawv qhov loj.
Ntxiv mus, lwm qhov sib txawv tseem ceeb ntawm tus nqi zonal thiab isopycnic centrifugation yog tias tus nqi zonal centrifugation yog nyob ntawm lub sijhawm, thaum lub isopycnic centrifugation yog ywj siab ntawm lub sijhawm. Dhau li ntawd, qhov gradient muab los ntawm tus nqi zonal centrifugation yog tiaj tus thaum nyob rau hauv isopycnic centrifugation nws yog ntxhab.
Summary – Tus Nqi Zonal vs Isopycnic Centrifugation
Centrifugation yog ib qho tseem ceeb analytical txheej txheem. Muaj ob hom kev centrifugation uas peb siv hauv kev siv tshuaj lom neeg: tus nqi zonal thiab isopycnic centrifugation. Qhov sib txawv tseem ceeb ntawm tus nqi zonal thiab isopycnic centrifugation yog tias tus nqi zonal centrifugation yog qhov tseem ceeb hauv kev sib cais cov khoom sib txawv ntawm qhov loj tab sis tsis nyob rau hauv lawv qhov ntom, qhov isopycnic centrifugation tseem ceeb hauv kev sib cais cov khoom sib txawv ntawm qhov ntom tab sis tsis nyob hauv lawv qhov loj.
