Qhov sib txawv tseem ceeb ntawm Giemsa Stain thiab Leishman Stain yog tias Giemsa staining yog qhov muaj txiaj ntsig zoo hauv cov xim ntawm DNA thaj tsam ntawm cov chromosomes sib txawv los tshawb xyuas qhov txawv txav xws li kev hloov pauv thiab kev hloov kho tshiab, thaum Leishman stain muaj txiaj ntsig zoo thaum cov ntshav smear staining thiab tsom xam. txhawm rau txheeb xyuas thiab txheeb xyuas cov trypanosomes, leukocytes thiab kab mob malaria.
Staining yog ib kauj ruam tseem ceeb thaum lub sij hawm txhim kho qhov sib piv ntawm cov duab microscopic nyob rau hauv cov ntsiab lus ntawm microscopy, tshwj xeeb tshaj yog hais txog cov qauv sib txawv hauv cov hlwb thiab cov ntaub so ntswg. Giemsa stain thiab Leishman stain belongs rau pawg Romanowsky stains, uas kuj suav nrog Wright stain thiab Jenner stain. Feem ntau, Romanowsky stains muaj txiaj ntsig zoo hauv kev ua kom cov ntshav smears. Peb tsuas yog siv qhov no thaum kawm txog cov ntshav liab morphology thiab kev ua tau zoo ntawm cov qe ntshav dawb sib txawv. Eosin Y thiab azure B dyes yog cov khoom siv rau Romanowsky stains. Romanowsky staining cov txheej txheem pab hauv kev kuaj mob ntawm cov kab mob sib txawv xws li Leukemia.
Giemsa Stain yog dab tsi?
Giemsa stain feem ntau yuav pab kom sib txawv ntawm cytoplasmic thiab nuclear morphology ntawm cov qe ntshav zoo li cov qe ntshav dawb, cov qe ntshav liab thiab platelets. Tsis tas li ntawd, nws yuav pab kom cov kab mob sib txawv. Feem ntau, nws yog siv rau cytogenetics thiab hauv kev kuaj mob malaria thiab lwm yam kab mob parasitic. Ntxiv mus, Giemsa stain yog tshwj xeeb rau phosphate pawg uas muaj nyob hauv DNA. Lawv ua raws li cov cheeb tsam uas muaj ntau dua ntawm adenine-thymine bonding hauv DNA strand.
Daim duab 01: Giemsa Stain
Tsis tas li ntawd, Giemsa stain tseem muaj txiaj ntsig hauv Giemsa banding lossis G banding kom stain chromosomes thiab tsim karyograms. Yog li ntawd, Giemsa stain muaj peev xwm txheeb xyuas thiab pom qhov txawv txav ntawm cov chromosomes. Piv txwv li, lub trophozoite ntawm Trichomonas vaginalis, uas muab tawm ib tug ntsuabish paug thiab muaj motile hlwb nyob rau hauv ntub npaj, yog stained nrog Giemsa stain. Raws li tau hais los saum toj no, Giemsa stain ua raws li cov ntshav zaj duab xis stain. Thaum stained, cov qe ntshav liab stains liab, platelets stain nyob rau hauv lub teeb los yog daj ntseg liab, thiab lub cytoplasm ntawm lymphocytes, monocytes thiab leukocytes stain nyob rau hauv lub ntuj xiav, daj ntseg xiav thiab magenta, feem.
Giemsa stain yog sib xyaw ntawm eosin, methylene xiav thiab Azure B. Cov methylene azure sib tov uas tsim ib qho eosinate nrog rau methylene xiav stabilizes qhov sib tov. Nyob rau hauv tus txheej txheem ntawm Giemsa stain, ib zaj duab xis nyias ntawm cov qauv yog muab tso rau ntawm ib qho chaw me me ua ntej. Cov kauj ruam tom ntej yog kho nrog ntshiab methanol li ntawm 30 vib nas this los ntawm kev ntxiv ob peb tee ntawm methanol ntawm tus swb. Tom qab ntawd, tus swb yog raus rau hauv 5% Giemsa stain tov rau li 20-30 feeb. Cov kauj ruam kawg yog ntxuav cov swb nrog cov kais dej thiab tawm mus kom qhuav.
Leishman Stain yog dab tsi?
Tus kws kho mob Scottish William Boog Leishman yog tus tsim tawm ntawm Leishman stain. Nws yog ib qho ntawm cov stains uas belongs rau pawg ntawm Romanowsky stains. Tsis tas li ntawd, nws tau siv ntau dua hauv kev sib txawv thiab kev txheeb xyuas cov kab mob malarial sib txawv, trypanosomes - unicellular flagellated protozoa, uas yog kab mob cab thiab leucocytes.
Daim duab 02: Leishman Stain
Lub hauv paus ntawm Leishman stain yog cov tshuaj methanolic uas muaj cov sib xyaw ntawm methylene xiav uas yog 'polychromed'; demethylated rau ntau hom azures thiab eosin. Vim muaj kev ruaj ntseg ntawm cov khoom lag luam ntawm cov tshuaj methanolic sib tov, peb tuaj yeem siv nws ncaj qha los kho cov smear, thaum tshem tawm cov kauj ruam ua ntej. Qhov kev ruaj ntseg yuav txo qis yog tias cov tshuaj tov nrog ib qho aqueous buffer. Thaum ua qhov sib txawv ntawm tes, Leishman stain muab cov yam ntxwv ci xim violet rau cov nucleus thiab neutrophil granules. Yog li, nws txhim kho qhov sib txawv ntawm cov nucleus thiab cytoplasm. Tsis tas li ntawd, Leishman stain muab ntau qhov zoo sib xws staining nyob rau hauv kev sib piv nrog rau lwm yam stains uas yog methylene xiav thiab eosin raws li.
Vim tias cov khoom sib txawv cytoplasmic tau hais meej meej rau kev sib txawv thiab kev txheeb xyuas, cov kws kho mob haematologist nyiam Leishman stain piv rau lwm cov Romanowsky stains. Hauv kev tshawb nrhiav cov kab mob malarial, Leishman staining cov txheej txheem yog rhiab thiab raug dua li lwm cov stains xws li Field's stain.
Dab tsi zoo sib xws ntawm Giemsa Stain thiab Leishman Stain?
- Giemsa stain thiab Leishman stain yog qhov sib txawv.
- Ob leeg muaj txiaj ntsig zoo thaum ua haujlwm ntawm qhov sib txawv ntawm cov qe ntshav dawb suav thiab kawm txog cell morphology ntawm cov qe ntshav liab.
- Ntxiv mus, ob qho tib si stains yog pawg ntawm Romanowsky stains.
Qhov txawv ntawm Giemsa Stain thiab Leishman Stain yog dab tsi?
Giemsa staining yog qhov muaj txiaj ntsig zoo hauv kev staining ntawm DNA thaj tsam ntawm cov chromosomes sib txawv los tshawb xyuas qhov txawv txav xws li kev hloov pauv thiab hloov kho, thaum Leishman stain muaj txiaj ntsig zoo hauv cov ntshav staining kom sib txawv thiab txheeb xyuas trypanosomes, leucocytes thiab kab mob malaria. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm Giemsa stain thiab Leishman stain.
Ntxiv mus, tus kws kho mob bacteriologist Gustav Giemsa tau tsim Giemsa staining txheej txheem, thaum tus kws kho mob William Boog Leishman tsim Leishman staining txheej txheem. Tsis tas li ntawd, lwm qhov sib txawv tseem ceeb ntawm Giemsa stain thiab Leishman stain yog qhov muaj pes tsawg leeg ntawm cov stain. Giemsa stain yog sib xyaw ntawm eosin, methylene xiav thiab Azure B thaum Leishman stain yog cov tshuaj methanolic uas muaj cov sib xyaw ntawm methylene xiav.
Summary – Giemsa Stain vs Leishman Stain
Nyob rau hauv cov ntsiab lus ntawm microscopy, staining cov tswv yim ua lub luag haujlwm tseem ceeb hauv kev txhim kho qhov sib piv ntawm cov duab microscopic ntawm ntau cov ntaub so ntswg lom. Giemsa staining yog qhov tseem ceeb hauv kev staining ntawm DNA thaj tsam ntawm cov chromosomes sib txawv los tshawb xyuas qhov txawv txav xws li kev hloov pauv thiab kev hloov kho tshiab. Leishman stain muaj txiaj ntsig zoo hauv cov ntshav smear staining thiab tshuaj xyuas kom sib txawv thiab txheeb xyuas cov trypanosomes, leucocytes, thiab malaria parasites. Yog li, qhov no yog qhov txawv ntawm Giemsa stain thiab Leishman stain.
