Qhov Tseem Ceeb Qhov txawv - Giemsa Stain vs Wright Stain
Nyob rau hauv cov ntsiab lus ntawm microscopy, staining yog suav hais tias yog ib qho tseem ceeb kauj ruam thaum txhim kho qhov sib piv ntawm cov duab microscopic, tshwj xeeb tshaj yog hais txog cov qauv sib txawv hauv cov ntaub so ntswg lom. Thaum lub sij hawm staining ntawm peripheral ntshav thiab pob txha pob txha smears, Wright thiab Giemsa stains yog siv. Cov stains no hu ua Romanowsky stains. Ob lub stains no yog tsim los ntawm cov khoom tseem ceeb: oxidized methylene xiav, eosin Y, thiab azure B dyes. Kev ua haujlwm ntawm methylene xiav thiab azure B yog kom stain lub nucleus nrog cov xim sib txawv ntawm xiav mus rau ntshav. Cov stains no tau siv dav hauv kev tshawb fawb ntawm cov qe ntshav liab morphology thiab thaum lub sij hawm kev ua haujlwm ntawm cov qe ntshav dawb sib txawv. Kev kuaj mob ntawm cov kab mob sib txawv xws li leukemia tuaj yeem ua tiav los ntawm Romanowsky staining txheej txheem. Wright staining yog siv los sib txawv cov qe ntshav uas muaj kev sib xyaw ntawm eosin thiab methylene xiav dyes. Giemsa staining yog siv thaum lub sij hawm staining ntawm cov kab mob hlwb nrog rau tib neeg lub hlwb thiab tuaj yeem ua ke nrog Wright stain los tsim Giemsa Wright stain. Qhov no yog qhov sib txawv tseem ceeb ntawm Giemsa stain thiab Wright stain.
Daim duab 01: Giemsa Stain
Giemsa tov muaj methylene xiav, Azure B thiab eosin thiab cov stain yog npaj ua lag luam nrog kev siv Giemsa hmoov. Kev ruaj ntseg ntawm cov stain nyob ntawm methylene azure thiab nws cov sib tov nrog methylene xiav uas tsim ib qho eosinate. Giemsa stain yog tshwj xeeb rau cov pab pawg phosphate hauv DNA strand, thiab nws tau txuas mus rau thaj chaw uas muaj cov khoom sib txuas ntawm adenine-thymine ntau. Nyob rau hauv Giemsa staining txoj kev, ib txheej nyias ntawm cov qauv yog muab tso rau thawj zaug rau ntawm ib tug microscopic swb nrog rau ob peb tee ntawm ntshiab methanol li ntawm 30 vib nas this. Tom qab ntawd cov swb yog raus rau hauv 5% Giemsa stain tov, uas yog npaj tshiab, rau li 20 - 30 feeb. Thaum kawg, tus swb yog ntxuav nrog kais dej thiab sab laug kom qhuav. Giemsa stain yog lub npe hu ua qhov sib txawv vim tias Wright's-Giemsa Stain yog tsim thaum Wright's stain tau ua ke nrog Giemsa. Yog li ntawd, nws tuaj yeem siv rau hauv kev tshawb fawb ntawm cov kab mob pathogenic txuas rau tib neeg lub hlwb. Ntawm no, tib neeg lub hlwb thiab cov kab mob cov kab mob yog stained deferentially thiab liab doog thiab liab xim yog pom raws.
Wright Stain yog dab tsi?
Wright's stain yog lub npe tom qab James Homer Wright uas hloov Romanowsky stain. Wright's stain yog siv los sib txawv ntawm cov qe ntshav vim nws pab kom paub qhov txawv ntawm hom qe ntshav. Yog li ntawd, cov kab mob tuaj yeem kuaj tau los ntawm kev soj ntsuam cov ntshav dawb suav. Cov stain yog sib xyaw ntawm eosin, uas yog xim liab, thiab methylene xiav dyes. Wright's stain yog siv los stain thiab soj ntsuam cov qauv tso zis, cov ntshav peripheral smears, thiab cov pob txha pob txha aspirates nyob rau hauv lub teeb tsom iav. Wright's stain yog siv rau hauv staining chromosomes hauv cytogenetics los txhawb kev kuaj mob ntawm ntau yam kab mob thiab cov tsos mob. Cov qauv zis uas muaj stained nrog Wright's stain qhia cov eosinophils qhia tias muaj kab mob hauv cov zis.
Daim duab 02: Wright Stain
Nyob rau hauv Wright tus txheej txheem stain, cov yeeb yaj kiab cua qhuav tau npaj thiab Wright stain yog thov thiab sab laug rau 3 feeb. Tom qab ntawd, qhov tsis muaj qhov sib npaug ntawm cov stain yog ntxiv, sib tov maj mam thiab sab laug rau 5 feeb. Lub swb yog tuav horizontally thiab ntxuav zoo nrog nruab nrab distilled dej. Thaum kawg, nws tau qhuav thiab pom nyob rau hauv lub microscope.
Dab tsi yog qhov zoo sib xws ntawm Giemsa stain thiab Wright stain?
- Ob lub stains no yog tsim los ntawm cov khoom tseem ceeb: oxidized methylene xiav, eosin Y, thiab azure B dyes.
- Ob leeg yog siv thaum lub sijhawm ua haujlwm ntawm qhov sib txawv ntawm cov qe ntshav dawb suav thiab kawm txog cell morphology ntawm cov qe ntshav liab.
- Ob leeg yog qhov sib txawv.
Qhov txawv ntawm Giemsa Stain thiab Wright Stain yog dab tsi?
Giemsa Stain vs Wright Stain |
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| Giemsa stain yog cov txheej txheem staining sib txawv siv feem ntau rau staining ntawm cov kab mob thiab cov hlwb tib neeg. | Wright stain yog txheej txheem staining sib txawv siv feem ntau hauv cov txheej txheem staining ntawm cov ntshav smears, kuaj zis, thiab cov pob txha pob txha aspirates. |
Summary – Giemsa Stain vs Wright Stain
Staining yog ib qho tseem ceeb hauv kev sim cov txheej txheem uas yog siv thaum lub sij hawm tsom xam uas yog siv los txhim kho qhov sib piv ntawm cov duab microscopic. Giemsa stain thiab Wright Stain ua ke hu ua Romanowsky stains koom nrog hauv kev ua haujlwm sib txawv ntawm cov qe ntshav dawb suav thiab kawm txog cell morphology ntawm cov qe ntshav liab. Oxidized methylene xiav, eosin Y, thiab azure B dyes yog cov khoom tseem ceeb ntawm Romanowsky stains. Feem ntau, Giemsa stain yog siv thaum lub sij hawm staining ntawm cov kab mob hlwb tab sis nws tuaj yeem siv rau tib neeg lub hlwb thiab. Wright staining yog dav siv thaum lub sij hawm staining ntawm cov ntshav smears, kuaj zis, thiab pob txha pob txha aspirates. Qhov no yog qhov txawv ntawm Giesma stain thiab Wright's stain.
Download PDF Version of Giemsa Stain vs Wright Stain
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version hereDifference between Giemsa Stain thiab Wright Stain
