Qhov sib txawv tseem ceeb ntawm kev thim rov qab thiab qhov tsis tuaj yeem thim rov qab yog qhov thim rov qab inhibition yog hom enzyme inhibition uas cuam tshuam ntawm inhibitor los ntawm cov enzyme-inhibitor complex yog ua tau vim tsis yog-covalent khi. Ntawm qhov tod tes, irreversible inhibition yog ib hom enzyme inhibition nyob rau hauv uas dissociation ntawm inhibitor los ntawm cov enzyme-inhibitor complex yog tsis tau vim covalent binding.
Enzymes yog cov proteins uas ua cov tshuaj lom neeg hauv peb lub cev. Lawv nce tus nqi ntawm cov tshuaj tiv thaiv. Substrates khi nrog cov chaw nquag ntawm cov enzymes thiab hloov mus rau hauv cov khoom. Txawm li cas los xij, enzymes tshwj xeeb rau cov substrate. Enzyme kev txiav txim tuaj yeem tswj hwm lossis cuam tshuam los ntawm qee yam inhibitors. Muaj ob hom enzyme inhibition txheej txheem; uas yog, lawv yog cov thim rov qab inhibition thiab irreversible inhibition. Nyob rau hauv reversible inhibition, tus inhibitor khi nrog cov enzyme uas tsis yog-covalently thaum nyob rau hauv lub irreversible inhibition, tus inhibitor khi nrog cov enzyme los ntawm covalently los yog non-covalently. Ob txheej txheem no txawv ntawm ib leeg, thiab kab lus no npaj siab los tham txog qhov sib txawv ntawm kev thim rov qab thiab qhov tsis tuaj yeem thim rov qab kom meej.
Yuav ua li cas thim rov qab Inhibition?
Hauv kev thim rov qab, tus inhibitor inactivates lub enzyme los ntawm kev khi tsis-covalently nrog nws. Yog li ntawd, qhov thim rov qab inhibition tsis yog kev sib cuam tshuam ntawm cov enzyme thiab inhibitor. Yog li, los ntawm kev nce cov concentration ntawm cov substrate, qhov no tuaj yeem rov qab tau yooj yim, thiab nws muaj peev xwm rov ua kom cov enzyme yooj yim dua. Ntxiv mus, muaj ob lub ntsiab ntawm kev thim rov qab inhibition txheej txheem; uas yog, lawv yog kev sib tw inhibition thiab tsis sib tw inhibition.
Hauv kev sib tw inhibition, inhibitor zoo li lub substrate, thiab nws sib tw nrog lub substrate rau qhov chaw nquag ntawm cov enzyme. Thaum tus inhibitor nyob ntawm qhov chaw nquag, lub substrate tsis tuaj yeem khi nrog cov enzyme, thiab cov tshuaj tiv thaiv tsis tshwm sim. Txawm li cas los xij, thaum lub substrate concentration siab, kev sib tw inhibition tuaj yeem tiv thaiv.
Daim duab 01: Rov qab inhibition
Ntawm qhov tod tes, nyob rau hauv kev sib tw tsis sib tw, inhibitor tsis zoo li lub substrate. Li no, nws tsis sib tw nrog lub substrate rau lub active site binding. Nws khi ntawm ib qho chaw sib txawv ntawm cov enzyme (allosteric site) thiab hloov cov qauv peb sab ntawm cov enzyme. Thaum cov qauv peb-dimensional ntawm cov enzyme hloov, nws cov haujlwm txo qis. Li no, cov tshuaj tiv thaiv tshwm sim ntawm tus nqi qeeb lossis nws tsis tshwm sim.
Dab tsi yog Kev Tiv Thaiv Tsis Txaus Siab?
Irreversible inhibition yog hom thib ob ntawm enzyme inhibition, uas inhibitor khi nrog cov enzyme los ntawm covalent daim ntawv cog lus muaj zog thiab inhibits cov enzyme kev ua. Yog li ntawd, nws yog ib qho nyuaj rau unbind lub inhibitor los ntawm lub enzyme. Yog li ntawd, nws tsis tuaj yeem thim rov qab cov tshuaj tiv thaiv. Irreversible inhibitors feem ntau muaj cov pab pawg ua haujlwm ua haujlwm. Yog li, lawv tuaj yeem khi nrog cov amino acid chains ntawm cov enzyme thiab tsim covalent bonds.
Daim duab 02: Irreversible Inhibition
Tsis tas li ntawd, irreversible inhibitors yog tshwj xeeb. Yog li ntawd, lawv tsis khi nrog tag nrho cov proteins. Qee qhov piv txwv ntawm cov inhibitors tsis tuaj yeem yog penicillin, aspirin, diisopropylfluorophosphate, thiab lwm yam. Muaj peb hom irreversible inhibitors; uas yog, lawv yog pab pawg tshwj xeeb reagents, substrate analogues, thiab suicide inhibitors.
Dab tsi yog qhov zoo sib xws ntawm Reversible thiab Irreversible Inhibition?
- thim rov qab thiab irreversible inhibition yog ob hom enzyme inhibition txoj hauv kev.
- Nyob rau hauv ob qho tib si, tus inhibitor khi nrog cov enzyme.
- Tsis tas li, ob qho tib si tuaj yeem hloov pauv kev ua haujlwm catalytic ntawm enzyme.
Dab tsi yog qhov txawv ntawm Reversible thiab Irreversible Inhibition?
thim rov qab inhibition thiab irreversible inhibition yog ob hom enzyme inhibition txoj hauv kev. Qhov sib txawv tseem ceeb ntawm kev thim rov qab thiab irreversible inhibition yog tias nws muaj peev xwm thim rov qab thim rov qab inhibition thaum nws tsis tuaj yeem thim rov qab qhov irreversible inhibition. Tsis tas li ntawd, nyob rau hauv reversible inhibition, tus inhibitor khi nrog cov enzyme los ntawm cov tsis muaj zog tsis muaj cov kev sib cuam tshuam thaum nyob rau hauv lub irreversible inhibition, lub inhibitor khi nrog cov enzyme los ntawm ib tug muaj zog covalent daim ntawv cog lus. Yog li ntawd, kev sib cais ntawm cov enzyme-inhibitor complex yog sai nyob rau hauv reversible inhibition thaum lub dissociation ntawm lub enzyme-inhibitor complex yog qeeb thiab nyuaj nyob rau hauv irreversible inhibition. Yog li, nws yog lwm qhov sib txawv ntawm kev thim rov qab thiab qhov tsis tuaj yeem thim rov qab.
Ntxiv mus, nyob rau hauv thim rov qab inhibition, thaum lub inhibitor tshem tawm, lub enzyme pib ua hauj lwm dua thaum nyob rau hauv lub irreversible inhibition, lub enzyme tsis pib ua hauj lwm dua txawm tias tus inhibitor tawm ntawm lub enzyme. Yog li, qhov no kuj yog qhov sib txawv ntawm kev thim rov qab thiab qhov tsis tuaj yeem inhibition. Tsis tas li ntawd, muaj ob lub ntsiab ntawm kev thim rov qab inhibition uas yog kev sib tw inhibition thiab tsis sib tw inhibition thaum muaj peb hom irreversible inhibition uas yog pab pawg tshwj xeeb reagents, substrate analogues, thiab suicide inhibitors.
Hauv qab no yog ib qho infographic ntawm qhov sib txawv ntawm kev thim rov qab thiab qhov tsis tuaj yeem thim rov qab.
Summary – Reversible vs Irreversible Inhibition
Enzyme inhibition tuaj yeem thim rov qab lossis rov tsis tau. Nyob rau hauv summarizing qhov txawv ntawm reversible thiab irreversible inhibition; Nyob rau hauv reversible inhibition, tus inhibitor khi nrog cov enzyme uas tsis yog-covalently. Yog li ntawd, lub unbinding ntawm inhibitor los ntawm lub enzyme yog yooj yim thiab ceev. Ntawm qhov tod tes, hauv irreversible inhibition, tus inhibitor khi nrog cov enzyme covalently. Yog li ntawd, tus inhibitor xav tau khi nrog cov enzyme thiab dissociation ntawm enzyme-inhibitor complex yog qeeb thiab nyuaj. Yog li ntawd, qhov no yog qhov sib txawv tseem ceeb ntawm kev thim rov qab thiab tsis muaj kev cuam tshuam. Tsis tas li ntawd, nyob rau hauv thim rov qab inhibition, cov tshuaj tiv thaiv tuaj yeem thim rov qab, thiab cov enzyme tuaj yeem rov ua haujlwm dua. Tab sis nyob rau hauv irreversible inhibition, cov tshuaj tiv thaiv tsis tuaj yeem thim rov qab, thiab cov enzyme tsis tuaj yeem rov ua dua.
