Qhov sib txawv tseem ceeb ntawm prokaryotic thiab eukaryotic topoisomerase yog lub hauv paus ntawm cov cellular ntawm topoisomerase. Prokaryotic topoisomerases muaj nyob rau hauv cov hlwb ntawm prokaryotic cellular keeb kwm thaum eukaryotic topoisomerases muaj nyob rau hauv cov kab mob nrog eukaryotic cellular keeb kwm. Tsis tas li ntawd, lawv txawv ntawm kev faib khoom thiab. Prokaryotic topoisomerases muaj nyob rau hauv cytoplasm ntawm lub cell thaum eukaryotic topoisomerases raug faib nyob rau hauv lub nucleus.
Isomerase yog ib pawg ntawm cov enzymes uas ua rau cov txheej txheem rov ua haujlwm ntawm cov molecules. Topoisomerase yog ib hom isomerases. Nws hloov cov topology ntawm DNA molecule los ntawm kev tswj DNA supercoiling. Nws txiav thiab rov ua dua ib lossis ob qho tib si ntawm DNA duplex. Yog li ntawd, DNA muaj peev xwm mus rau replication, transcription, kho thiab chromosomal cais. Muaj ob hom topoisomerases uas yog topoisomerase I thiab II.
Prokaryotic Topoisomerase yog dab tsi?
Prokaryotic DNA Topoisomerases yog cov enzymes xav tau thaum prokaryotic DNA replication. Lawv txo cov kev ntxhov siab thaum lub sij hawm DNA supercoiling los ntawm ua rau ib leeg-stranded thiab ob-stranded so.
Hom Prokaryotic Topoisomerases
Type I topoisomerases yog lub luag haujlwm rau ib leeg-stranded so whereas Type II topoisomerases ua rau ob-stranded so. Topo IA, Topo IC, thiab Reverse Gyrase yog peb hom prokaryotic topoisomerases uas feem ntau muaj nyob rau hauv cov kab mob thiab archaea. Hom IIA thiab Hom IIB yog hom II topoisomerases tam sim no hauv prokaryotes.
Camptothecin thiab non-Camptothecin inhibit qhov kev ua ntawm prokaryotic topoisomerase hom I, thiab lawv yog cov npe nrov tshuaj rau kev kho mob qog noj ntshav.
YEukaryotic Topoisomerase yog dab tsi?
Eukaryotic Topoisomerases koom nrog hauv eukaryotic DNA replication. Lawv pab tshem tawm qhov zoo thiab tsis zoo supercoils thaum lub sij hawm unwinding ntawm ob lub helix ntawm qhov pib theem ntawm replication
Hom Eukaryotic Topoisomerases
Eukaryotes nqa ob hom I thiab hom II topoisomerases. Zoo ib yam li prokaryotes, hom I topoisomerases tawg ib leeg ntawm DNA. Nyob rau hauv sib piv, hom II topoisomerases ua rau ob-stranded so. Hom I topoisomerases nyob rau hauv eukaryotes yog cov subgroups ntawm hom IB topoisomerase, whereas hom IIA subclasses nrog rau hom IIα muaj nyob rau hauv siab dua eukaryotes xws li tsiaj txhu. Cov poov xab muaj qhov tshwj xeeb topoisomerases.
Daim duab 01: Topoisomerase Action hauv Eukaryotes
Camptothecin thiab cov tshuaj uas tsis yog-camptothecin tuaj yeem cuam tshuam eukaryotic topoisomerases ib yam nkaus. Yog li, lawv muaj txiaj ntsig zoo li cov tshuaj tiv thaiv kab mob los tiv thaiv kev loj hlob ntawm cov qog nqaij hlav cancer los ntawm inhibiting cov txheej txheem rov ua dua.
Dab tsi yog qhov zoo sib xws ntawm Prokaryotic thiab Eukaryotic Topoisomerase?
- Ob qho tib si topoisomerases ua rau ib leeg stranded los yog ob zaug stranded so kom txo tau qhov kev ntxhov siab thaum lub sij hawm supercoiling.
- Lawv yog qhov tseem ceeb rau DNA replication.
- Lawv tuaj yeem tshem tawm ob qho tib si zoo thiab tsis zoo supercoils.
- Ob lub topoisomerases muaj ob hom xws li hom I thiab hom II.
- Camptothecin thiab cov tshuaj uas tsis yog-camptothecin inhibit qhov kev ua ntawm ob qho tib si topoisomerases.
Qhov txawv ntawm Prokaryotic thiab Eukaryotic Topoisomerase yog dab tsi?
Prokaryotic topoisomerases muaj nyob rau hauv cytoplasm ntawm cov kab mob thiab archaea. Nyob rau hauv sib piv, Eukaryotic topoisomerases muaj nyob rau hauv lub nucleus. Topo IA, Topo IC, thiab Reverse Gyrase yog hom I prokaryotic topoisomerase thaum hom I eukaryotic topoisomerase suav nrog cov pab pawg ntawm hom IB topoisomerase. Hom II prokaryotic topoisomerase suav nrog Hom IIA thiab Hom IIB thaum hom II eukaryotic topoisomerase suav nrog hom IIA subclasses.
Summary – Prokaryotic vs Eukaryotic Topoisomerase
DNA topoisomerases yog cov enzymes uas koom nrog hauv kev tshem tawm qhov zoo thiab tsis zoo supercoils tsim thaum lub sij hawm unwinding txheej txheem ntawm DNA replication. Qhov txawv ntawm prokaryotic thiab eukaryotic topoisomerase nyob ntawm lawv lub hauv paus chiv keeb ntawm cov enzyme thiab kev faib tawm.
