Qhov sib txawv tseem ceeb ntawm cov khoom siv thiab cov kua dej yog cov khoom siv xov xwm muaj agar thaum cov kua dej tsis muaj agar. Ntawd yog, agar yog tus neeg sawv cev khov kho hauv kev tshaj tawm kev loj hlob, thiab cov khoom siv xov xwm muaj cov neeg sawv cev khov kho thaum cov kua xov xwm tsis muaj tus neeg sawv cev solidifying. Ntxiv rau qhov no, hais txog kev siv, qhov sib txawv ntawm cov khoom siv thiab cov kua dej tawm yog cov khoom siv tau siv rau kev cais cov kab mob los yog txiav txim siab cov yam ntxwv ntawm cov kab mob. Txawm li cas los xij, cov kua dej siv rau ntau lub hom phiaj xws li kev nthuav tawm ntau yam kab mob, kev tshawb fawb fermentation, thiab ntau yam kev sim.
Cov kab mob microorganisms tau cog thiab khaws cia hauv cov chaw kuaj mob microbiological rau ntau lub hom phiaj. Kev loj hlob ntawm cov kab mob yog ua tiav los ntawm kev loj hlob nruab nrab, thiaj li yuav tau txais kev loj hlob txaus. Kev loj hlob nruab nrab lossis kab lis kev cai nruab nrab yog txhais tau tias yog cov khoom tsim los yog kua uas muaj cov as-ham thiab lwm yam khoom tsim nyog los txhawb kev loj hlob ntawm cov kab mob thiab cov hlwb. Cov xov xwm loj hlob tuaj yeem yog cov khoom siv los yog ua kua.
Dab tsi yog Media?
Kev loj hlob xov xwm yog tsim raws li kev loj hlob ntawm cov kab mob thiab lub hom phiaj ntawm kev loj hlob microbes ntawm kev tshaj tawm. Kab lis kev cai xov xwm muaj cov khoom noj tsim nyog rau lwm cov khoom xyaw uas xav tau rau cov kab mob. Muaj ntau hom kab lis kev cai xov xwm. Cov kab lis kev cai feem ntau tau npaj ua cov qauv tsim los ntawm kev ntxiv cov neeg sawv cev solidification (agar) feem ntau ntawm qhov concentration ntawm 1.5%. Agar yog ib yam khoom zoo li jelly uas yog siv los ua kom muaj zog nruab nrab. Nws yog ib qho inert tshuaj uas tsis muaj zaub mov muaj nqis. Agar yog muab rho tawm los ntawm ntau hom liab algae. Kev lag luam agar yog muab los ntawm Gelidium liab algae. Khoom nruab nrab yog sib tov ntawm agar thiab lwm yam as-ham. Thaum cov agar ntxiv lawm, qhov nruab nrab yuav khov rau ntawm chav tsev kub.
Cov xov xwm muaj zog feem ntau yog nchuav rau hauv cov tais diav los npaj cov phaj agar. Lub agar phaj muab qhov chaw zoo thiab qhov chaw rau cov kab mob aerobic, tshwj xeeb tshaj yog cov kab mob thiab cov fungi, kom loj hlob zoo. Cov phaj agar nrog cov kab mob no tuaj yeem siv los kawm lawv cov yam ntxwv. Cov kab mob microbes uas yuav tsum tau siv rau cov txheej txheem staining yog loj hlob hauv cov khoom siv xov xwm ntawm daim hlau. Cov yam ntxwv morphological kuj tuaj yeem pom los ntawm cov kab mob loj hlob ntawm agar daim hlau. Cov khoom siv xov xwm tseem siv los npaj agar slants kom loj hlob cov kab mob rau lub hom phiaj cia.
Daim duab 01: Khoom Media
Liquid Media yog dab tsi?
Cov kua dej tawm yog hom kab lis kev cai siv los cog thiab tswj cov kab mob. Lawv kuj raug hu ua kab lis kev cai broths. Cov kua dej tawm tsis tau ntxiv nrog tus neeg sawv cev solidifying. Yog li ntawd, cov xov xwm no nyob twj ywm li kua txawm nyob rau hauv chav tsev kub. Cov xov xwm ua kua feem ntau yog nchuav rau hauv cov raj xa dej los yog lub raj mis kab lis kev cai.
Thaum cov kab mob loj hlob hauv cov hlab ntsha, lawv raug cais raws li qhov xav tau oxygen. Yog li, cov kab mob loj hlob ntawm cov hlab ntsha yog siv los sib txawv cov kab mob raws li lawv cov kev xav tau oxygen. Cov kab mob uas xav tau cov pa oxygen (qhib aerobes) yuav loj hlob nyob ze ntawm qhov nruab nrab ntawm cov kua txiv hmab txiv ntoo thaum cov kab mob uas muaj tshuaj lom rau cov pa oxygen (cov anaerobes nruj) yuav loj hlob nyob rau hauv qab ntawm lub raj dej. Cov kab mob uas tuaj yeem nyob hauv lub xub ntiag thiab tsis muaj oxygen yog hu ua facultative anaerobes, thiab lawv pom feem ntau nyob rau saum lub raj. Cov kab mob microaerophilic muaj nyob rau sab sauv ntawm lub raj dej, tab sis tsis nyob rau saum toj. Cov kab mob Aerotolerant feem ntau loj hlob sib npaug thoob plaws hauv lub raj dej.
Daim duab 02: Kua Media
Dab tsi yog qhov sib txawv ntawm Cov Khoom Siv thiab Kua Media?
Solid vs Liquid Media |
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| Khoom siv xov xwm yog hom kab lis kev cai siv los cog cov kab mob. | Cov kua dej yog hom kab lis kev cai siv los cog cov kab mob. |
| Muaj Agar | |
| Solid media contain agar. | Kua media tsis muaj agar. |
| Petri lauj kaub | |
| Cov khoom siv khoom yog nchuav rau hauv cov tais diav. | Kua xov xwm tsis nchuav rau hauv cov tais diav. |
| Cov khoom siv yog siv los cais cov kab mob los yog txiav txim siab cov yam ntxwv ntawm cov kab mob. |
Cov kua dej yog siv rau ntau lub hom phiaj xws li kev nthuav tawm ntau cov kab mob, kev tshawb fawb fermentation, thiab ntau yam kev sim. Eg. Kev kuaj qab zib fermentation, MR-VR broth |
Summary – Khoom vs Liquid Media
Cov khoom siv thiab cov kua dej yog ob hom kab lis kev cai uas siv ntau tshaj plaws. Cov khoom nruab nrab yog npaj los ntawm kev ntxiv ib qho khoom solidifying. Feem ntau siv cov khoom ua kom muaj zog yog gelatin lossis agar. Cov xov xwm ua kua tsis yog muab nrog tus neeg sawv cev solidifying. Ob qho tib si khoom thiab kua xov xwm muaj cov as-ham thiab lwm yam khoom tsim nyog los txhawb kev loj hlob ntawm cov kab mob. Qhov sib txawv tseem ceeb ntawm cov khoom siv thiab cov kua dej yog qhov muaj lossis tsis muaj agar lossis tus neeg sawv cev solidifying.
Download PDF Version of Solid vs Liquid Media
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li cov ntawv sau tseg. Thov rub tawm PDF version ntawm no Qhov txawv Ntawm Cov Khoom Siv thiab Kua Media
