Qhov Tseem Ceeb Qhov txawv – Ntawv vs Nyias Txheej vs Kab Chromatography
Ntawv chromatography, nyias txheej chromatography, thiab kem chromatography yog peb hom kev kawm chromatographic. Qhov sib txawv tseem ceeb ntawm daim ntawv chromatography, nyias txheej chromatography thiab kem chromatography yog raws li hom kev nyob ruaj khov uas siv rau hauv cov txheej txheem chromatography. Daim ntawv chromatography siv daim ntawv cellulose raws li nws cov theem nyob ruaj khov, Nyias Txheej chromatography siv alumina los yog silica gel raws li nws cov theem nyob ruaj khov, whereas Kem chromatography siv ib kem ntim nrog cov khoom siv matrix haum raws li nws cov theem nyob ruaj khov.
Nyob rau hauv cov txheej txheem ntawm kev sib cais thiab txheeb xyuas cov biomolecules xws li cov protein thiab carbohydrates, chromatography yog ib qho tseem ceeb biophysical txheej txheem siv. Chromatography cais cov tebchaw raws li lawv cov solubility, loj thiab nqi. Raws li cov txheej txheem sib cais, chromatography siv cov txheej txheem xws li kev sib pauv ion, nqus, muab faib thiab qhov loj me thiab tsis suav nrog peb cov txheej txheem chromatographic; uas yog, ntawv, nyias txheej, thiab kab chromatography. Daim ntawv chromatography yog raws li cov khoom-kua adsorption thiab solubility ntawm cov compound, thiab nws siv ib daim ntawv cellulose raws li cov theem nyob ruaj khov. Nyias txheej chromatography yog raws li cov khoom-kua adsorption ntawm molecules. Nws muaj cov theem nyob ruaj khov uas feem ntau yog ua los ntawm alumina lossis silica gel thiab lub sijhawm txawb uas yog qhov hnyav. Kem chromatography siv ib kem ntim nrog cov matrix uas yog siv los cais cov molecules feem ntau raws li lawv qhov loj me, affinity lossis nws cov nqi.
Daim Ntawv Chromatography yog dab tsi?
Daim ntawv chromatography yog hom chromatography yooj yim tshaj plaws siv, thiab nws tsis yog siv rau kev tshawb fawb dav. Nws yog tsuas yog siv nyob rau hauv cov tub ntxhais kawm lub chaw soj nstuam los txheeb xyuas biomolecules xws li amino acids thiab carbohydrates uas muaj nyob rau hauv mixs. Daim ntawv chromatography siv lub sij hawm nyob ruaj ruaj uas yog tsim los ntawm cov ntawv cellulose los yog Whatman lim ntawv thiab cov theem ntawm lub xov tooj ntawm tes uas feem ntau yog npaj siv cov kuab tshuaj organic xws li n-butanol, thiab lwm yam. Cov theem nyob ruaj khov yog saturated nrog dej, ua rau cov kua theem nyob ruaj khov. Yog li, thaum lub tebchaw pom thiab tso cai rau khiav hauv lub xub ntiag ntawm lub xov tooj ntawm tes, nyob ntawm seb lub solubility ntawm cov tebchaw, lawv raug cais. Yog li, raws li kev txhim kho ntawm chromatogram, staining tuaj yeem ua tiav los txiav txim siab qhov ntev ntawm txhua qhov sib xyaw. Qhov ntsuas khaws cia tuaj yeem suav tau.
Daim duab 01: Daim Ntawv Chromatography
Paper chromatography tuaj yeem raug cais ntxiv raws li ascending paper chromatography thiab descending paper chromatography nyob ntawm seb cov kev taw qhia ntawm cov kuab tshuaj khiav.
Thiab Txheej Chromatography yog dab tsi?
Thin txheej chromatography lossis TLC yog cov txheej txheem siv los txheeb xyuas cov amino acids sib txawv hauv cov sib tov lossis rau kev txheeb xyuas cov protein. Cov txheej txheem ntawm kev sib cais yog raws li cov khoom-kua adsorption. Thaum lub sij hawm nyias txheej chromatography, ib lub phaj ua los ntawm alumina los yog silica gel yog siv raws li theem nyob ruaj ruaj. Cov kuab tshuaj sib tov sib txawv raws li qhov yuav tsum tau ua thiab tuaj yeem siv sib txawv ntawm cov organic sib xyaw xws li n-butanol, acetic acid, thiab dej los npaj cov kuab tshuaj. Cov tebchaw yuav tsum tau sib cais yog pom ntawm lub phaj thiab muab tso rau hauv qhov sib tov hnyav. Thaum cov kuab tshuaj mus txog raws li qhov kev txiav txim ntawm capillary muab los ntawm lub phaj, cov tebchaw pom ntawm lub phaj kuj txav nyob ntawm lawv cov solubility hauv cov kuab tshuaj.
Daim duab 02: Nyias Txheej Chromatography
Kev kuaj pom ntawm cov pob tom qab chromatogram tau ua tiav los ntawm cov txheej txheem staining sib txawv. Qee tus siv ninhydrin staining uas yog ib txoj hauv kev zoo rau staining. Niaj hnub nyias txheej chromatograms siv cov txheej txheem fluorescence los saib cov chromatogram tom qab khiav. Nyob ntawm qhov deb nws tau mus ncig, lub sijhawm khaws cia ntawm txhua qhov sib xyaw tuaj yeem suav tau. Qhov no tuaj yeem siv los txheeb xyuas hom kev sib xyaw sib cais raws li kev sib xyaw siv. TLC feem ntau yog siv los txheeb xyuas cov amino acids hauv cov protein sib xyaw thiab tseem cais ntau hom monosaccharides tam sim no hauv kev sib tov.
Kem Chromatography yog dab tsi?
Kem chromatography yog ib lo lus dav siv los piav txog ntau hom chromatography cov txheej txheem uas siv kab raws li txoj kev sib cais. Hauv kem chromatography, ib kem lub cev yog siv nrog cov khoom ntim los cais cov tebchaw. Kev sib cais yuav ua raws li cov khoom sib txawv ntawm lub cev uas nthuav tawm los ntawm cov tebchaw. Cov khoom no tuaj yeem yog tus nqi, qhov loj me, 3D conformation thiab binding muaj peev xwm, thiab lwm yam. Yog li, kem ntim nrog cov khoom siv matrix ua raws li theem nyob ruaj khov thiab cov ntxhua khaub ncaws tsis siv rau hauv kem ua raws li theem mobile.
Yog tias cov molecules raug cais raws li qhov loj me, cov khoom ntim tau ntim rau hauv ib qho uas nws tawm qhov pores rau cov tebchaw mus los. Yog li, cov molecules loj dua uas tsis tuaj yeem ntws los ntawm qhov pores yog eluted ua ntej, whereas cov me me molecules siv sij hawm ntev dua los elute.
Daim duab 03: Kem Chromatography
Yog tias cov molecules raug cais raws li lawv cov nqi, cov theem nyob ruaj khov yuav muaj cov anion lossis cation exchanger uas cov tebchaw yuav tau txais kev nyiam raws li lawv cov nqi. Yog li thaum lub sij hawm ntxuav, cov khoom tsis sib txuas yuav raug tshem tawm. Thaum ntxiv cov elution tsis, cov bound charged compounds yuav eluted. Kev tshawb pom ntawm cov eluents no feem ntau yog raws li cov txheej txheem spectrophotometric.
Dab tsi yog qhov zoo sib xws ntawm Daim Ntawv Nyias Txheej thiab Kem Chromatography?
- Txhua daim ntawv nyias txheej thiab kab chromatography peb cov txheej txheem yog siv rau kev sib cais ntawm biomolecules xws li amino acids, proteins thiab carbohydrates.
- Cov txheej txheem ntawv nyias nyias thiab kab chromatography cov txheej txheem muaj cov theem txawb thiab theem nyob ruaj khov.
- Daim Ntawv nyias txheej thiab kab chromatography cov txheej txheem siv cov txheej txheem biophysical rau kev sib cais.
Dab tsi yog qhov txawv ntawm daim ntawv nyias txheej thiab kab chromatography?
Paper vs Nyias txheej vs Kem Chromatography |
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| Paper Chromatography | Daim ntawv chromatography yog cov txheej txheem chromatographic siv los cais cov tebchaw raws li cov kua-kua adsorption thiab solubility ntawm cov compound. Nws siv daim ntawv cellulose raws li nws cov theem nyob ruaj khov. |
| Thin Layer Chromatography | Nyias txheej chromatography yog lwm cov txheej txheem chromatographic raws li cov kua-kua adsorption ntawm molecules. Nws muaj cov theem nyob ruaj ruaj ua los ntawm alumina los yog silica gel thiab hnyav raws li lub sijhawm txawb, uas yog cov kuab tshuaj. |
| Kab Chromatography | Kem chromatography siv ib kem ntim nrog cov matrix uas yog siv los cais cov molecules feem ntau raws li lawv qhov loj me, affinity lossis nws tus nqi. |
| Paper Chromatography | Daim ntawv ua los ntawm nitrocellulose ntawm Whatman yog siv los ua theem nyob ruaj khov hauv ntawv chromatography. |
| Thin Layer Chromatography | Alumina lossis Silica gel yog siv los ua cov theem ntawm cov txheej nyias nyias chromatography. |
| Kab Chromatography | Ib kem ntim nrog cov khoom ntim tsim nyog yog siv los ua theem nyob ruaj khov hauv kab chromatography. |
| Mob Phase | |
| Paper Chromatography | Khiav hnyav yog theem mobile ntawm daim ntawv chromatography. |
| Thin Layer Chromatography | Khiav hnyav yog lub sijhawm txawb ntawm cov txheej nyias nyias chromatography. |
| Kab Chromatography | Ntxuav tsis yog lub sijhawm txawb ntawm kab chromatography. |
| Mechanisms Siv rau kev sib cais | |
| Paper Chromatography | Daim ntawv chromatography yog raws li cov khoom-kua nqus. |
| Thin Layer Chromatography | Nyias txheej chromatography yog raws li cov khoom siv-kua nqus. |
| Kab Chromatography | Kem chromatography yog raws li qhov loj me, nqi thiab cov duab. |
| Elution Buffer | |
| Paper Chromatography | Tsis xav tau los ntawm daim ntawv chromatography. |
| Thin Layer Chromatography | Tsis tas rau nyias txheej chromatography. |
| Kab Chromatography | Yuav tsum tau nyob hauv kab chromatography. |
| Ntseeg | |
| Paper Chromatography | Staining thiab los ntawm kev txiav txim siab txog qhov Retention factor. |
| Thin Layer Chromatography | Staining thiab los ntawm kev txiav txim siab txog qhov Retention factor. |
| Kab Chromatography | kev txiav txim siab saib xyuas. |
Summary – Ntawv nyias txheej vs Kem Chromatography
Daim ntawv chromatography, TLC thiab kem chromatography yog cov txheej txheem sib cais siv los cais cov biomolecules xws li cov proteins, amino acids thiab carbohydrates (feem ntau monosaccharides). Daim ntawv chromatography siv daim ntawv cellulose raws li cov theem nyob ruaj khov, thiab cov txheej txheem ntawm kev sib cais yog raws li cov khoom siv-kua adsorption. TLC kuj tseem siv cov khoom siv ua kua adsorption. Cov molecules yog sib cais nyob rau theem nyob ruaj khov, nyob ntawm seb lawv solubility nyob rau hauv lub mobile theem. Kem chromatography siv lub cev muaj zog xws li qhov loj me, zoo li, tus nqi thiab qhov hnyav molecular ntawm cov khoom sib cais. Lub kem ntim nrog cov khoom siv matrix ua raws li theem nyob ruaj khov, qhov chaw ntxhua khaub ncaws ua raws li cov theem hnyav. Qhov no yog qhov txawv ntawm daim ntawv nyias txheej thiab kab chromatography.
