Qhov sib txawv tseem ceeb ntawm fluorescence microscopy thiab confocal microscopy yog tias nyob rau hauv fluorescence microscopy, tag nrho cov qauv yog dej nyab sib npaug hauv lub teeb los ntawm lub teeb ci, whereas hauv confocal microscopy, tsuas yog qee cov ntsiab lus ntawm cov qauv raug pom los ntawm lub teeb. lub teeb ci.
Fluorescence microscopy yog ib qho tseem ceeb analytical txheej txheem uas yuav pab tau nyob rau hauv kev kawm cov khoom ntawm cov organic los yog inorganic tshuaj. Confocal microscopy yog cov txheej txheem kev tshuaj xyuas muaj txiaj ntsig hauv kev txhim kho qhov kho qhov muag thiab qhov sib txawv ntawm lub micrograph siv lub spatial pinhole los thaiv cov teeb pom kev tawm hauv cov duab tsim.
Fluorescence Microscopy yog dab tsi?
Fluorescence microscopy yog ib qho tseem ceeb analytical txheej txheem uas yuav pab tau nyob rau hauv kev kawm cov khoom ntawm cov organic los yog inorganic tshuaj. Cov cuab yeej uas peb siv rau qhov ntsuas no yog fluorescence microscope. Nws yog ib hom optical microscope. Hom tshuaj kho qhov muag no siv fluorescence es tsis txhob tawg, kev xav, attenuation, thiab nqus los yog ntxiv rau lawv.
A fluorescence microscope siv fluorescence los tsim cov duab. Tej zaum nws yuav yog ib qho kev teeb tsa yooj yim xws li lub tshuab hluav taws xob epifluorescence lossis ib qho kev tsim nyuaj dua, suav nrog lub tshuab tsom iav confocal uas siv cov khoom siv kho qhov muag. Qhov no yuav pab kom tau txais kev daws teeb meem zoo dua ntawm cov duab fluorescence.
Daim duab 01: A Fluorescence Microscope
Thaum xav txog lub hauv paus ntsiab lus ntawm fluorescence microscopy, peb yuav tsum siv cov qauv rau illumination nrog lub teeb ntawm ib lub wavelength tshwj xeeb. Nyob ntawd, cov qauv absorbs lub teeb los ntawm fluorophores uas ua rau lawv emit lub teeb ntawm ntev wavelengths. Nws yog ib qho tseem ceeb uas yuav tsum tau cais lub teeb pom kev los ntawm qhov tsis muaj zog emission fluorescence los ntawm kev siv lub spectral emission filter.
Feem ntau, lub tshuab hluav taws xob fluorescence muaj lub teeb pom kev zoo, lub zog lim dej, daim iav dichroic, thiab cov lim dej tawm. Lub teeb ci tuaj yeem yog xenon arc teeb, mercury-vapor teeb, LEDs, thiab lasers. Daim iav dichroic tseem hu ua dichroic beamsplitter.
Confocal Microscopy yog dab tsi?
Confocal microscopy yog ib qho kev tshuaj ntsuam xyuas muaj txiaj ntsig hauv kev ua kom qhov kev daws teeb meem kho qhov muag thiab qhov sib piv ntawm micrograph siv lub spatial pinhole los thaiv lub teeb pom kev hauv cov duab tsim. Nws tseem hu ua confocal laser scanning microscopy lossis laser confocal scanning microscopy. Nws yog cov txheej txheem kho qhov muag pom.
Nyob hauv cov txheej txheem microscopic no, ntes ntau cov duab 2D ntawm qhov tob sib txawv hauv cov qauv ua rau kev tsim kho ntawm 3D qauv hauv ib yam khoom. Cov txheej txheem confocal microscopic yog qhov tseem ceeb hauv kev tshawb fawb thiab kev lag luam hauv zej zog. Nws feem ntau yog siv hauv kev tshawb fawb txog lub neej, kev tshuaj xyuas semiconductor, thiab cov ntaub ntawv science.
Daim duab 02: Lub Confocal Point Sensor Txoj Cai los ntawm Minsky's Patent
Thaum lub sijhawm ua haujlwm, lub teeb mus los ntawm cov qauv hauv qab lub tshuab ntsuas pa kom deb li deb mus rau hauv cov qauv raws li nws tuaj yeem nkag mus, thaum lub tshuab tsom iav confocal uas tsuas yog tsom rau lub teeb me me ntawm lub teeb dhau los ntawm qhov tob tob hauv ib lub sijhawm. Cov txheej txheem no tau tsim los ntawm Marvin Minsky hauv xyoo 1957.
Qhov txawv ntawm Fluorescence Microscopy thiab Confocal Microscopy yog dab tsi?
Fluorescence microscopy yog ib qho tseem ceeb analytical txheej txheem uas yuav pab tau nyob rau hauv kev kawm cov khoom ntawm cov organic los yog inorganic tshuaj. Confocal microscopy yog cov txheej txheem kev tshuaj xyuas muaj txiaj ntsig hauv kev txhim kho qhov kho qhov muag thiab qhov sib txawv ntawm lub micrograph siv lub spatial pinhole los thaiv cov teeb pom kev tawm hauv cov duab tsim. Qhov sib txawv tseem ceeb ntawm fluorescence microscopy thiab confocal microscopy yog tias nyob rau hauv fluorescence microscopy, tag nrho cov qauv yog dej nyab tusyees nyob rau hauv lub teeb los ntawm lub teeb qhov chaw, whereas nyob rau hauv confocal microscopy, tsuas yog ib co ntsiab lus ntawm cov specimen raug rau lub teeb los ntawm lub teeb qhov chaw.
Cov duab hauv qab no nthuav tawm qhov sib txawv ntawm fluorescence microscopy thiab confocal microscopy hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Fluorescence Microscopy vs Confocal Microscopy
Fluorescence microscopy thiab confocal microscopy yog ob txoj kev tshuaj ntsuam xyuas koom nrog hauv kev kho qhov muag. Qhov sib txawv tseem ceeb ntawm fluorescence microscopy thiab confocal microscopy yog tias nyob rau hauv fluorescence microscopy, tag nrho cov qauv yog dej nyab tusyees nyob rau hauv lub teeb los ntawm lub teeb qhov chaw, whereas nyob rau hauv confocal microscopy, tsuas yog ib co ntsiab lus ntawm cov specimen raug rau lub teeb los ntawm lub teeb qhov chaw.
