Qhov sib txawv tseem ceeb ntawm kev nqus thiab fluorescence yog tias peb tuaj yeem siv cov txheej txheem kev ntsuas kev nqus dej kom ncaj qha ntsuas tus nqi ntawm ib lub wavelength tshwj xeeb uas tau nqus los ntawm tus qauv yam tsis muaj dilution lossis kev tshuaj ntsuam xyuas, qhov kev tshuaj ntsuam fluorescence xav tau cov qauv npaj nyob rau hauv uas Cov qauv ntawm kev txaus siab yuav tsum tau khi nrog cov fluorescent reagents nyob rau hauv cov khoom siv tshuaj ntsuam.
Absorbance thiab fluorescence yog cov txheej txheem tshuaj ntsuam xyuas tseem ceeb uas peb tuaj yeem siv los txheeb xyuas cov khoom sib txawv hauv cov qauv muab.
Dab tsi yog Absorbance?
Absorbance yog ib qho kev ntsuas ntawm lub peev xwm ntawm ib yam khoom los nqus lub teeb ntawm ib lub wavelength. Tshwj xeeb, nws yog sib npaug rau lub logarithm ntawm lub reciprocal ntawm transmittance. Tsis zoo li optical ntom ntom, absorbance ntsuas qhov ntau ntawm lub teeb absorbed los ntawm ib yam khoom.
Tsis tas li ntawd, spectroscopy ntsuas absorbance (siv colorimeter lossis spectrophotometer). Lub absorbance yog ib tug dimensionless khoom, tsis zoo li feem ntau lwm yam khoom ntawm lub cev. Muaj ob txoj hauv kev los piav txog kev nqus dej: raws li lub teeb nqus los ntawm cov qauv lossis raws li lub teeb kis los ntawm cov qauv. Qhov sib npaug rau kev suav ntawm absorbance yog raws li nram no:
A=log10(I0/I)
Qhov twg A yog nqus, I0 yog cov hluav taws xob xa tawm los ntawm cov qauv, thiab kuv yog qhov xwm txheej hluav taws xob. Cov kab zauv hauv qab no kuj zoo ib yam li cov kab zauv saum toj no hais txog kev xa tawm (T).
A=-log10T
Fluorescence yog dab tsi?
Fluorescence yog qhov emission ntawm lub teeb los ntawm cov khoom uas tau nqus lub zog yav dhau los. Cov tshuaj no yuav tsum nqus lub teeb lossis lwm yam hluav taws xob hluav taws xob kom tawm lub teeb ua fluorescence. Tsis tas li ntawd, lub teeb emission no yog hom luminescence, txhais tau tias nws emits spontaneously. Lub teeb emitted feem ntau muaj lub wavelength ntev dua li lub teeb absorbed. Qhov ntawd txhais tau hais tias lub teeb hluav taws xob emitted qis dua lub zog nqus.
Thaum lub sijhawm txheej txheem ntawm fluorescence, lub teeb raug tawm los ntawm kev ua kom muaj zog ntawm atoms hauv cov khoom. Lub zog absorbed feem ntau tso tawm raws li luminescence nyob rau hauv lub sij hawm luv luv, txog 10-8 vib nas this. Qhov ntawd txhais tau hais tias peb tuaj yeem soj ntsuam fluorescence sai li sai tau thaum peb tshem tawm cov hluav taws xob uas ua rau muaj kev ntxhov siab.
Muaj ntau daim ntawv thov ntawm fluorescence nyob rau hauv ntau qhov chaw, xws li mineralogy, gemology, tshuaj, tshuaj sensors, biochemical tshawb fawb, dyes, biological detectors, fluorescent teeb tsim, thiab lwm yam. Ntxiv mus, peb tuaj yeem pom cov txheej txheem no raws li cov txheej txheem ntuj tsim thiab; Piv txwv li, nyob rau hauv ib co minerals.
Qhov txawv ntawm Absorbance thiab Fluorescence yog dab tsi?
Absorbance thiab fluorescence yog cov txheej txheem tshuaj ntsuam xyuas tseem ceeb uas peb tuaj yeem siv los txheeb xyuas cov khoom sib txawv hauv cov qauv muab. Qhov sib txawv tseem ceeb ntawm absorbance thiab fluorescence yog tias peb tuaj yeem siv cov txheej txheem kev ntsuas kev nqus los ntsuas ncaj qha rau tus nqi ntawm cov wavelength tshwj xeeb uas tau nqus los ntawm cov qauv yam tsis muaj dilution lossis kev tshuaj ntsuam xyuas, qhov kev tshuaj ntsuam fluorescence xav tau cov qauv npaj hauv cov qauv kev txaus siab yuav tsum yog. khi nrog cov fluorescent reagents nyob rau hauv ib qho khoom kuaj. Tsis tas li ntawd, cov txheej txheem fluorescence yog qhov ua tau zoo dua li kev nqus dej vim tias qhov kev ntsuam xyuas hauv fluorescence yog qhov tshwj xeeb heev rau lub hom phiaj ntsuas.
Cov hauv qab no qhia txog qhov sib txawv ntawm qhov nqus thiab fluorescence hauv daim ntawv tabular rau ib sab ntawm kev sib piv
Summary – Absorbance vs Fluorescence
Absorbance yog ib qho kev ntsuas ntawm lub peev xwm ntawm ib yam khoom los nqus lub teeb ntawm lub wavelength uas tau teev tseg. Fluorescence yog lub emission ntawm lub teeb los ntawm ib yam khoom uas tau absorbed zog yav dhau los. Qhov sib txawv tseem ceeb ntawm absorbance thiab fluorescence yog tias peb tuaj yeem siv cov txheej txheem kev ntsuas absorbance los ntsuas tus nqi ntawm cov wavelength tshwj xeeb uas yog absorbed los ntawm cov qauv yam tsis muaj dilution los yog assay npaj, whereas fluorescence tsom xam yuav tsum tau ua qauv npaj nyob rau hauv cov qauv ntawm kev txaus siab yuav tsum tau. khi nrog cov fluorescent reagents nyob rau hauv ib qho khoom kuaj.
