Qhov sib txawv tseem ceeb ntawm PCNA thiab Ki67 yog PCNA yog cov protein ntau nuclear uas koom nrog DNA synthesis thiab kho txoj hauv kev, thaum Ki67 yog cov protein ntau nuclear uas koom nrog hauv cell proliferation thiab ribosomal RNA transcription.
Cell proliferation yog ib txoj kev lom neeg uas tseem ceeb rau txhua yam kab mob nyob. Kev tswj ntawm cov txheej txheem no yog dysregulated kiag li nyob rau hauv qee hom qog nqaij hlav. Yog li ntawd, kev ntsuam xyuas ntawm cell proliferation hauv cov qog tau dhau los ua ib qho cuab yeej nrov hauv kev kuaj mob qog. Nws kuj pab xaiv txoj kev kho kom tsim nyog rau cov qog. Cell proliferation markers yog cov proteins tshwj xeeb uas nws muaj nyob rau hauv nquag loj hlob thiab faib cov hlwb ua qhov taw qhia rau cov hlwb. PCNA thiab Ki67 yog ob lub cim kev loj hlob ntawm tes uas nquag siv los kuaj mob qog.
PCNA yog dab tsi?
Proliferating cell nuclear antigen (PCNA) yog DNA clamp-type protein uas ua haujlwm rau DNA polymerase delta hauv eukaryotic hlwb. Nws yog ib qho tseem ceeb heev rau DNA replication. Cov protein no yog ib qho protein ntau uas tseem ua lub luag haujlwm tseem ceeb hauv kev kho DNA. PCNA muaj homotrimer qauv. Nws ib txwm ua tiav nws cov txheej txheem los ntawm kev ncig ntawm DNA, qhov uas nws ua raws li lub scaffold los nrhiav lwm cov proteins koom nrog hauv DNA replication, DNA kho, chromatin remodeling, thiab epigenetics.
Daim duab 01: PCNA
No nuclear protein muaj ob lub ntsiab tseem ceeb: PCNA interacting peptide (PIP) lub thawv thiab AlkB homolog 2 PCNA interacting motif (APIM). Ntau cov proteins cuam tshuam nrog PCNA ntawm cov npe no. Cov proteins uas khi rau PCNA ntawm lub thawv PIP tau koom nrog hauv DNA replication. Ntawm qhov tod tes, cov proteins uas khi rau PCNA ntawm APIM yog koom nrog kev ntxhov siab genotoxic. Ntxiv mus, cov protein no tau nce siab thaum lub sijhawm G1 / S ntawm lub voj voog ntawm tes. Cov hlwb Quiescent muaj qis heev ntawm PCNA. Yog li, PCNA qhia tuaj yeem siv los ua tus cim ntawm kev loj hlob ntawm tes vim tias cov hlwb tseem nyob ntev hauv G1 / S theem thaum proliferating.
Ki67 yog dab tsi?
Ki67 yog ib qho protein ntau hauv cov cell proliferation thiab ribosomal RNA transcription. Nws tseem hu ua antigen Ki-67 lossis MKI67. Nws yog nuclear protein encoded los ntawm MK167 noob hauv tib neeg. Qhov no nuclear protein yog txuam nrog cellular proliferation. Nws kuj tseem cuam tshuam nrog ribosomal RNA transcription. Qhov inactivation ntawm KI67 yog lub luag haujlwm rau inhibition ntawm ribosomal RNA synthesis. Ntxiv mus, Ki67 yog ib tug classical marker rau cellular proliferation.
Daim duab 02: Ki67
Thaum lub sijhawm sib cuam tshuam, ki67 antigen tuaj yeem kuaj pom hauv lub cell nucleus, thaum nyob hauv mitosis, nws tau tsiv mus rau saum npoo ntawm chromosomes. Tsis tas li ntawd, ki67 muaj nyob rau txhua lub sijhawm ua haujlwm ntawm lub voj voog ntawm tes (G1, S, G2, thiab mitosis). Txawm li cas los xij, nws tsis muaj nyob rau hauv so quiescent hlwb. Hauv kev mob qog noj ntshav mis, qhov kev sim ki67 immunohistochemistry tuaj yeem tshawb pom sai npaum li cas cov qog nqaij hlav hauv lub mis faib. Hauv kev mob qog noj ntshav ntawm lub mis, qhov kev sim ki67 txheeb xyuas qhov kev loj hlob ntawm cov neeg mob uas muaj ER-zoo mob qog noj ntshav uas tuaj yeem tau txais txiaj ntsig ntau dua los ntawm kev siv tshuaj khomob.
Dab tsi yog qhov zoo sib xws ntawm PCNA thiab Ki67?
- PCNA thiab Ki67 yog ob lub xovtooj ntawm tes uas nquag siv los kuaj cov qog.
- Ob leeg cov proteins tau nthuav tawm ntau hauv cov hlwb proliferating.
- Cov proteins no tuaj yeem kuaj pom hauv G1 thiab S theem ntawm lub voj voog ntawm tes.
- Lawv yog cov proteins nuclear uas tsim los ntawm cov amino acids.
- Lawv qhia tuaj yeem kuaj pom los ntawm immunohistochemistry.
Qhov txawv ntawm PCNA thiab Ki67 yog dab tsi?
PCNA yog cov protein ntau nuclear koom nrog hauv DNA synthesis thiab kho txoj hauv kev, thaum Ki67 yog cov protein nuclear koom nrog hauv cell proliferation thiab ribosomal RNA transcription. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm PCNA thiab Ki67. Tsis tas li ntawd, PCNA gene codes rau PCNA hauv tib neeg, thaum MK167 noob codes rau Ki67 hauv tib neeg.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm PCNA thiab Ki67 hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – PCNA vs Ki67
Kev ua haujlwm loj ntawm cov qog hlwb yog ib qho tseem ceeb prognostic cim nyob rau hauv kev kuaj mob cancer. PCNA thiab Ki67 yog ob feem ntau siv cell proliferation markers hauv kev kuaj mob qog. PCNA yog ib qho protein ntau hauv kev koom tes hauv DNA synthesis thiab kho txoj hauv kev, thaum Ki67 yog cov protein ntau hauv cov cell proliferation thiab ribosomal RNA transcription. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm PCNA thiab Ki67.
