Qhov sib txawv tseem ceeb ntawm Ki67 thiab BrdU yog tias Ki67 yog cov protein tshwj xeeb uas feem ntau siv hauv kev tshawb nrhiav cov cell proliferating hauv cov ntaub so ntswg nyob, thaum BrdU (bromodeoxyuridine) yog ib qho hluavtaws nucleoside feem ntau siv hauv kev tshawb nrhiav cov hlwb hauv cov neeg nyob. ntaub so ntswg.
Cell proliferation nce cov cell. Nws feem ntau txhais los ntawm qhov sib npaug ntawm kev faib tawm ntawm tes thiab kev poob ntawm tes los ntawm kev tuag ntawm tes lossis kev sib txawv. Hauv cov qog, cell proliferation yog nce. Cell proliferation yuav ntsuas los ntawm cov tebchaw los yog dyes incorporated rau hauv cov cell nyob. Kev loj hlob ntawm tes kuj tuaj yeem ntsuas los ntawm kev txheeb xyuas qhov qhia ntawm qee cov proteins hu ua proliferation markers hauv cov cell nyob. Ki67 thiab BrdU yog ob hom ntawm cov cim kev loj hlob siv los kuaj xyuas cov cell proliferation.
Ki67 yog dab tsi?
Ki67 yog cov protein tshwj xeeb uas feem ntau siv los kuaj pom cov cell proliferating hauv cov ntaub so ntswg. Nws tseem hu ua antigen-Ki67 lossis MKI67. Tib neeg cov noob MKI67 codes cov protein no. Cov protein no tseem cuam tshuam nrog ribosomal RNA transcription. Inactivation ntawm antigen Ki67 ua rau inhibition ntawm ribosomal RNA synthesis. Ki67 yog cov protein nyob hauv cov hlwb uas nce ntxiv thaum lub hlwb npaj los faib rau hauv cov hlwb tshiab. Cov txheej txheem staining tuaj yeem ntsuas qhov feem pua ntawm cov qog hlwb uas zoo rau KI67. Lub xub ntiag ntawm cov hlwb zoo dua qhia tias cov hlwb sib faib thiab tsim cov hlwb tshiab sai dua. Hauv kev mob qog noj ntshav mis, qhov tshwm sim ntawm tsawg dua 10% suav tias yog qis, 10-20% ciam teb, thiab siab yog ntau tshaj 20%.
Daim duab 01: Ki67
Ki67 yog tam sim no nyob rau txhua lub sijhawm ua haujlwm ntawm lub voj voog ntawm tes (G1, S, G2, thiab M). Tab sis nws tsis nyob hauv cov hlwb so (G0). Yog li ntawd, cov lus qhia nuclear ntawm Ki67 tuaj yeem raug soj ntsuam los ntsuas cov qog proliferation los ntawm immunohistochemistry (IHC). Ntxiv mus, nws yog ib tug nrov proliferation marker niaj hnub siv nyob rau hauv pathology labs vim nws muaj peev xwm kuaj.
BrdU yog dab tsi?
BrdU (bromodeoxyuridine) yog ib qho hluavtaws nucleoside feem ntau siv hauv kev tshawb nrhiav cov cell proliferating hauv cov ntaub so ntswg. Yuav kom loj hlob, hlwb yuav tsum tsim DNA thaum lub sij hawm S. Yog li ntawd, ib txoj kev txhim khu kev qha rau kev ntsuam xyuas cell proliferation yog ntsuas ntawm DNA synthesis. Feem ntau, qhov no yog ua los ntawm incubating nyob hlwb nrog cov tebchaw los yog dyes uas tau yooj yim muab tso rau hauv tshiab synthesized DNA.
Daim duab 02: BrdU
Thymidine analogues yog cov khoom siv nrov tshaj plaws rau kev koom ua ke rau hauv DNA. Yog li ntawd, BrdU yog ib qho thymidine analogue uas feem ntau siv rau hauv kev tshawb nrhiav cov cell proliferating nyob rau hauv cov ntaub so ntswg. BrdU tuaj yeem muab tso rau hauv cov khoom tsim tshiab DNA ntawm replicating hlwb thaum lub sij hawm S. Qhov no yog vim nws hloov thymidine thaum lub sij hawm DNA replication. Kev tshawb nrhiav IHC ntawm BrdU kev koom ua ke rau hauv DNA yog cov txheej txheem muaj zog los kawm txog cytokinetic ntawm ib txwm thiab qog hlwb. Ntxiv mus, BrdU daim ntawv lo ntawm cov qog hlwb koom nrog cov kauj ruam xws li kev koom ua ke ntawm BrdU rau hauv DNA thiab kev tshawb pom tshwj xeeb ntawm kev koom ua ke BrdU nrog cov tshuaj tiv thaiv BrdU, thiab lwm yam.
Dab tsi yog qhov zoo sib xws ntawm Ki67 thiab BrdU?
- Ki67 thiab BrdU yog ob hom kev loj hlob ntawm cov cim siv hauv kev tshawb pom ntawm cell proliferation.
- Ob lub cim tuaj yeem siv los sib txawv ntawm cov qog nqaij hlav thiab cov qog.
- Lawv tuaj yeem sau cov hlwb hauv theem S.
- Ob lub cim qhia cov qauv qhia zoo sib xws.
- Lawv qhia kev ntseeg siab.
Qhov txawv ntawm Ki67 thiab BrdU yog dab tsi?
Ki67 yog cov protein tshwj xeeb uas feem ntau siv hauv kev tshawb nrhiav cov cell proliferating nyob rau hauv cov ntaub so ntswg nyob, thaum BrdU yog ib qho hluavtaws nucleoside uas feem ntau siv los kuaj xyuas cov cell proliferating hauv cov ntaub so ntswg. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm Ki67 thiab BrdU. Ntxiv mus, Ki67 sau cov hlwb hauv G1, G2, S thiab M theem ntawm lub voj voog ntawm tes thaum BrdU sau cov hlwb tsuas yog nyob rau theem S ntawm lub voj voog ntawm tes.
Cov hauv qab no infographic teev qhov sib txawv ntawm Ki67 thiab BrdU hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – Ki67 vs BrdU
Ki67 thiab BrdU yog ob hom kev loj hlob ntawm cov cim uas muaj txiaj ntsig zoo hauv kev tshawb nrhiav cov cell proliferation. Ki67 yog cov protein tshwj xeeb, thaum BrdU yog hluavtaws nucleoside. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm Ki67 thiab BrdU. Ki67 tuaj yeem sau cov hlwb hauv G1, G2, S thiab M theem ntawm lub voj voog ntawm tes. Hauv qhov sib piv, BrdU sau cov hlwb nkaus xwb nyob rau theem S ntawm lub voj voog ntawm tes.
