Qhov sib txawv tseem ceeb ntawm recombinant DNA thiab recombinant protein yog tias recombinant DNA yog ib tug molecule ntawm DNA uas yog tsim los ntawm kev coj cov noob caj noob ces los ntawm ntau hom thiab muab tso rau hauv ib tug tswv tsev kab mob los tsim cov noob caj noob ces tshiab, thaum recombinant protein. yog cov protein uas tau muab txhais los ntawm cov kab mob hauv lub cev raws li cov ntaub ntawv tam sim no hauv DNA recombinant.
Recombinant DNA thev naus laus zis suav nrog kev hloov pauv ntawm cov khoom siv caj ces sab nraum lub cev kom tau txais cov yam ntxwv xav tau hauv cov kab mob muaj sia lossis lawv cov khoom. Lub tshuab ntawm cov thev naus laus zis no suav nrog kev tso cov khoom tawg ntawm DNA los ntawm ntau qhov chaw uas muaj cov noob uas xav tau los ntawm kev tsim vector. Yog li ntawd, recombinant DNA thiab recombinant protein yog ib qho tseem ceeb ntawm recombinant DNA technology.
DNA Recombinant yog dab tsi?
Recombinant DNA yog ib qho molecule ntawm DNA ua los ntawm kev coj cov khoom siv caj ces los ntawm ntau hom sib txawv kom tsim tau cov caj ces tshiab. Cov kev sib txuas caj ces tshiab no muaj txiaj ntsig zoo rau kev tshawb fawb, tshuaj, kev ua liaj ua teb, thiab kev lag luam. Recombinant DNA kuj txhais tau tias yog ib daim DNA uas tau tsim los ntawm kev sib txuas yam tsawg kawg yog ob qho DNA ntawm ob qhov sib txawv. Recombinant DNA yog ua tau vim DNA molecules ntawm tag nrho cov kab mob sib koom cov qauv tshuaj. Lawv txawv tsuas yog nyob rau hauv lub nucleotide ib theem zuj zus nyob rau hauv uas zoo tib yam tag nrho cov qauv.
Daim duab 01: Recombinant DNA
Recombinant DNA molecule qee zaum hu ua chimeric DNA molecule vim lawv tuaj yeem ua los ntawm cov ntaub ntawv los ntawm ob hom sib txawv. Thaum tsim cov DNA molecules recombinant, piv txwv li, cog DNA tuaj yeem koom nrog cov kab mob DNA, lossis tib neeg DNA tuaj yeem koom nrog fungal DNA. Tsis tas li ntawd, DNA uas tsis tshwm sim nyob qhov twg hauv qhov xwm txheej tuaj yeem koom ua ke rau hauv cov molecules recombinant tom qab cov tshuaj synthesis hauv chav kuaj. Tsis tas li ntawd, qhov kev qhia ntawm cov DNA recombinant coded rau txawv teb chaws protein yuav tsum tau siv ib tug tshwj xeeb qhia vector thiaj li yuav qhia nyob rau hauv lub host organism.
Recombinant Protein yog dab tsi?
Recombinant protein yog hais txog cov protein uas tau txhais los ntawm tus tswv tsev kab mob raws li cov ntaub ntawv muaj nyob hauv DNA recombinant. Kev hloov pauv ntawm DNA los ntawm kev siv thev naus laus zis DNA tuaj yeem ua rau kev nthuav tawm ntawm cov protein sib xyaw. Recombinant protein yog ib daim ntawv manipulative ntawm ib txwm haiv neeg protein. Nws yog tsim nyob rau hauv ntau txoj kev nyob rau hauv thiaj li yuav nce zus tau tej cov proteins thiab tsim cov khoom lag luam muaj txiaj ntsig. Kev tsim cov protein ntau recombinant pib ntawm qib genetic uas cov txheej txheem coding rau cov protein ntawm kev txaus siab yuav tsum tau muab cais ua ntej thiab tom qab ntawd cloned rau hauv kev qhia plasmid vector. Tom qab ntawd, nws tau nkag mus rau hauv cov kab mob hauv lub cev los ntawm kev qhia vector. Ces tus tswv tsev tsim cov protein uas txaus siab.
Daim duab 02: Recombinant Protein
Ntau cov proteins sib xyaw ua ke yuav tsum tau hloov pauv cov protein xws li glycosylation. Poov xab, kab kab, kab lis kev cai mammalian cell kab lis kev cai ib txwm muaj xws li post translational modifications. Recombinant proteins muaj kev siv sib txawv. Lawv tau siv rau hauv kev tshuaj xyuas enzymatic xws li ELISA, western blot, thiab immunohistochemistry (IHC). Tsis tas li ntawd, lawv kuj tau siv hauv biotherapeutics xws li ntshav qab zib, mob qog noj ntshav, kab mob sib kis, haemophilia, thiab ntshav ntshav. Biotherapeutics recombinant proteins muaj xws li cov tshuaj tiv thaiv, Fc fusion proteins, cov tshuaj hormones, interleukins, enzymes, thiab anticoagulants.
Dab tsi zoo sib xws ntawm Recombinant DNA thiab Recombinant Protein?
- Recombinant DNA thiab recombinant protein yog tsim los ntawm recombinant DNA technology.
- Lawv tsis yog haiv neeg molecules.
- Lawv yog recombinant molecules.
- Ob leeg xav tau kev pab ntawm ib qho kev qhia tsim nyog vector.
- Lawv cov ntawv thov muaj txiaj ntsig zoo hauv kev tshawb fawb niaj hnub, tshuaj, kev ua liaj ua teb, thiab kev lag luam.
Qhov txawv ntawm Recombinant DNA thiab Recombinant Protein yog dab tsi?
Recombinant DNA yog ib qho molecule ntawm DNA ua los ntawm kev sib txuas cov khoom siv caj ces los ntawm ntau hom kev tsim cov caj ces tshiab. Recombinant protein yog hais txog cov protein uas tau muab txhais los ntawm cov kab mob hauv lub cev raws li cov ntaub ntawv muaj nyob hauv DNA recombinant. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm recombinant DNA thiab recombinant protein. Tsis tas li ntawd, recombinant DNA yog tsim los ntawm molecular cloning nyob rau hauv lub laboratory, thaum recombinant protein yog tsim nyob rau hauv lub ntuj host cell. Yog li, qhov no yog lwm qhov sib txawv ntawm recombinant DNA thiab recombinant protein. Recombinant DNA yog tsim los ntawm nucleotides, thaum recombinant protein yog tsim los ntawm cov amino acids.
Cov infographic hauv qab no teev qhov sib txawv ntawm cov DNA recombinant thiab recombinant protein nyob rau hauv daim ntawv tabular.
Summary – Recombinant DNA vs Recombinant Protein
Recombinant DNA thev naus laus zis txhais tau tias yog kev sib koom ua ke ntawm DNA molecules los ntawm cov kab mob sib txawv thiab muab tso rau hauv cov kab mob hauv lub cev txhawm rau tsim cov kev sib txuas caj ces tshiab uas muaj txiaj ntsig zoo hauv kev tshawb fawb, tshuaj, kev ua liaj ua teb, thiab kev lag luam. Yog li ntawd, recombinant DNA thiab recombinant protein yog ib qho tseem ceeb ntawm recombinant DNA technology. Recombinant DNA yog ib qho molecule ntawm DNA uas yog tsim los ntawm kev coj ua ke cov khoom siv caj ces los ntawm ntau hom tsiaj uas tau muab tso rau hauv cov kab mob hauv lub cev los tsim cov kev sib txuas caj ces tshiab, thaum cov protein recombinant hais txog cov protein uas tau txhais los ntawm cov kab mob hauv lub cev raws li cov ntaub ntawv tam sim no. nyob rau hauv lub recombinant DNA. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm DNA recombinant thiab recombinant protein.
