Qhov sib txawv tseem ceeb ntawm ssRNA thiab dsRNA yog tias ssRNA tsuas muaj ib txoj hlua ntawm RNA xwb thaum dsRNA yog tsim los ntawm ob qho sib ntxiv ntawm siRNA lossis miRNA strands.
RNA lossis ribonucleic acid yog ib hom nucleic acid uas yog tsim los ntawm ribonucleotides. Feem ntau, RNA yog ib leeg-stranded molecule, tsis zoo li DNA ob helix. Hauv B altimore kev faib tawm cov kab mob, Pawg III, Pawg IV thiab Pab Pawg V suav nrog cov kab mob RNA. Qee cov kab mob muaj ssRNA lossis ib leeg-stranded RNA genome. Yog li, lawv cov genome yog tsim los ntawm ib txoj hlua ntawm RNA. Ntxiv mus, qee cov kab mob muaj dsRNA lossis ob-stranded RNA genome. Yog li ntawd, lawv cov genome yog tsim los ntawm ob lub ntsiab lus ntawm RNA. Sucrose lossis cesium chloride density gradient txheej txheem tuaj yeem cais cov kab mob ssRNA thiab dsRNA cov kab mob. Cov khoom sib zog yog cov kab mob ssRNA, thaum cov khoom denser muaj dsRNA.
ssRNA yog dab tsi?
ssRNA sawv rau ib leeg-stranded RNA. Feem ntau, RNA yog ib leeg-stranded. Muaj cov kab mob muaj ssRNA genome. Lawv yog ob hom: kev nkag siab zoo thiab kev nkag siab tsis zoo raws li kev nkag siab lossis polarity ntawm RNA. Kev nkag siab zoo ib leeg-stranded RNA coj zoo li mRNA. Li no, nws tuaj yeem txhais ncaj qha rau hauv cov protein. Kev nkag siab tsis zoo ssRNA yog ntxiv rau mRNA. Li no, nws yuav tsum tau hloov mus rau hauv qhov zoo-sensitive ssRNA los ntawm RNA nyob ntawm RNA polymerase. Tom qab ntawd, nws tuaj yeem txhais ua cov protein.
Daim duab 01: RNA Viruses
Hauv B altimore kev faib tawm, kev nkag siab zoo ssRNA cov kab mob muaj nyob rau hauv Pawg IV thaum cov kab mob tsis zoo ssRNA koom nrog Pawg V. Hauv sucrose lossis cesium chloride density gradients, cov khoom sib zog muaj cov kab mob ssRNA.
DsRNA yog dab tsi?
dsRNA sawv rau ob-stranded RNA. Hauv dsRNA, muaj ob qhov sib ntxiv ntawm RNA. Cov strands no ua ke nrog ib leeg los ntawm kev tsim cov ntawv cog lus ntawm cov hauv paus ntxiv. Tsis zoo li ssRNA, dsRNA tsis ntev - nws yog luv luv molecule. dsRNA yog tsim thaum ntxiv DNA strands tau transcribed rau hauv RNA los ntawm symmetrical transcription los ntawm kev tawm tsam txhawb nqa. Ntxiv mus, ssRNA tuaj yeem tsim intra-strand ob chav helixes uas yog dsRNA los ntawm kev sib koom ua ke ua ke. dsRNA kuj tseem tuaj yeem tsim los ntawm kev sib koom ua ke ntawm ssRNAs uas ua los ntawm cov transposons thiab cov noob rov ua dua.
Daim duab 02: dsRNA Virus
Qee tus kab mob muaj dsRNA genomes. Cov kab mob no muaj ntau tus tswv tsev. Rotaviruses thiab picobirnaviruses yog cov kab mob dsRNA. Lub dsRNA genome ntawm cov kab mob no tau hloov mus rau hauv mRNA los ntawm RNA nyob ntawm RNA polymerase. Tom qab ntawd, mRNA molecules hloov mus rau cov kab mob kis kab mob. Tsis tas li ntawd, qhov kev nkag siab zoo RNA strand dua tuaj yeem siv los tsim dsRNA genomes rau cov kab mob tshiab. Hauv B altimore kev faib tawm, dsRNA belongs rau Pawg III. Cov kab mob dsRNA kis tau rau txhua yam kab mob xws li tsiaj txhu, kab mob, cov nroj tsuag thiab fungi.
Kev cuam tshuam me me RNA lossis siRNA yog hom dsRNA uas tuaj yeem ua rau RNA cuam tshuam hauv eukaryotes, nrog rau cov lus teb interferon hauv vertebrates.
Dab tsi zoo sib xws ntawm ssRNA thiab dsRNA?
- Muaj cov kab mob muaj ssRNA thiab dsRNA.
- Ib leeg-stranded RNA molecule tuaj yeem ua tiav kev sib koom ua ke los tsim cov intrastrand ob helixes uas yog dsRNA.
- Ob leeg ssRNA thiab dsRNA yog tsim los ntawm ribonucleotides muaj plaub lub hauv paus; adenine (A), cytosine (C), guanine (G), lossis uracil (U), ribose qab zib thiab phosphate pawg.
Qhov txawv ntawm ssRNA thiab dsRNA yog dab tsi?
Qhov sib txawv tseem ceeb ntawm ssRNA thiab dsRNA yog tias ssRNNA tsuas muaj ib txoj hlua xwb, thaum dsRNA muaj ob lub RNA txuas ntxiv ua ke ua ke. Hauv qhov xwm txheej, ssRNA pom muaj ntau thaum dsRNA tsawg dua. Hauv B altimore kev faib tawm, cov kab mob ssRNA nyob hauv Pawg IV thiab V, thaum cov kab mob dsRNA nyob hauv Pawg III.
Cov duab hauv qab no qhia qhov txawv ntawm ssRNA thiab dsRNA hauv daim ntawv tabular.
Summary – ssRNA vs dsRNA
Nyob rau hauv xwm, RNA pom raws li ib leeg-stranded molecule uas yog folded rau nws tus kheej. mRNA yog ib leeg-stranded RNA molecule uas txhais ua cov protein ntawm ribosomes. Ntau tus kab mob muaj RNA genomes. Qee cov kab mob muaj ssRNA genomes thaum qee cov kab mob muaj dsRNA genomes. dsRNA muaj ob lub RNA txuas ntxiv ua ke ua ke. Kev nkag siab zoo ssRNA ncaj qha ua raws li mRNA thiab txhais ua cov kab mob kis tau thaum lub siab tsis zoo ssRNA hloov mus rau qhov zoo-sens ssRNA los ntawm RNA nyob ntawm RNA polymerase thiab tom qab ntawd muab txhais ua cov protein. dsRNA tau hloov mus rau hauv mRNA los ntawm RNA nyob ntawm RNA polymerase, thiab tom qab ntawd cov mRNA molecules txhais ua cov kab mob kis. Yog li, qhov no qhia txog qhov sib txawv ntawm ssRNA thiab dsRNA.
