Qhov sib txawv tseem ceeb ntawm sucrose gradient thiab sucrose cushion ultracentrifugation yog tias nyob rau hauv sucrose gradient ultracentrifugation, ib qho txuas ntxiv sucrose gradient yog siv, thaum nyob rau hauv sucrose cushion ultracentrifugation, discontinuous sucrose gradient yog siv.
Sucrose gradient thiab sucrose cushion ultracentrifugation yog ob yam zoo sib xws ntawm cov txheej txheem siv los cais qee hom macromolecules. Nyob rau hauv ob txoj kev, ib tug sucrose density gradient yog siv. Tab sis nyob rau hauv sucrose gradient ultracentrifugation, ib tug nruam ceev gradient yog siv thaum, nyob rau hauv sucrose cushion ultracentrifugation, ib tug discontinuous ceev gradient yog siv.
Sucrose Gradient Ultracentrifugation yog dab tsi?
Sucrose gradient ultracentrifugation yog cov txheej txheem uas tuaj yeem siv los faib cov macromolecules xws li DNA, RNA thiab cov proteins. Muaj ob peb kauj ruam hauv cov txheej txheem no. Lawv yog sucrose gradient npaj, centrifugation, sib cais thiab elution. Kev npaj gradient yog kauj ruam tseem ceeb hauv cov txheej txheem no. Hauv cov txheej txheem no, cov qauv uas muaj qhov sib txawv me me macromolecules yog txheej txheej ntawm sucrose gradient kem. Thaum centrifuging, sib txawv me me macromolecules sediment los ntawm sucrose gradient kem ntawm tus nqi sib txawv. Sedimentation nyob ntawm ntau yam, suav nrog lub zog centrifugation, qhov loj me thiab qhov ntom ntawm macromolecules, ntom thiab viscosity ntawm gradient. Thaum kawg ntawm qhov centrifugation, macromolecules tau sib cais los ntawm qhov ceev. Loj macromolecules (high-density macromolecules) sediment rau hauv qab. Cov sib dua (cov macromolecules tsawg-density) nyob rau saum cov gradient. Yog li ntawd, cov molecules cais raws li sib txawv bands. Tom qab ntawd cov hlua yuav tsum tau sib cais, thiab kev ua kom huv ntawm cov macromolecule tshwj xeeb los ntawm sucrose yuav tsum tau ua.
Daim duab 01: Sucrose Gradient Ultracentrifugation
Ntawm daim ntawv thov, txoj kev no yog siv dav rau kev faib cov DNA molecules. Sucrose gradient ultracentrifugation feem ntau yog siv los qhia cov protein ntau thiab muaj pes tsawg leeg. Ntxiv mus, cov txheej txheem no yog siv rau kev ua kom huv ib nrab ntawm mRNA.
Sucrose Cushion Ultracentrifugation yog dab tsi?
Sucrose cushion ultracentrifugation yog lwm cov txheej txheem uas ua rau cov fractionation ntawm macromolecules. Tsis zoo li sucrose density gradient ultracentrifugation, sucrose cushion ultracentrifugation siv ib qho kev sib txuas tsis tu ncua. Sucrose concentration nce los ntawm sab saum toj mus rau hauv qab nyob rau hauv discrete kauj ruam. Nyob rau hauv cov txheej txheem no, kev sib cais yog ua tiav los ntawm kev tso cov ntshiab extract rau sab saum toj ntawm ib tug me me ntim ntawm ib tug sucrose tov nyob rau hauv ib tug centrifuge raj, lub thiaj li hu ua sucrose cushion. Qhov no tso cai rau kev sib cais zoo dua hauv kev kub ceev centrifugation. Thaum cov molecules sib cais raws li ib pab pawg ntawm sucrose cushion, lub band dawb tuaj yeem sau thiab ua kom huv. Ntxiv mus, impurities tuaj yeem raug tshem tawm ntxiv siv txoj kev sucrose cushion. Sucrose cushion txoj kev tso cai rau 60-70% ntawm lub raj kom puv nrog cov qauv. Li no, txoj kev sucrose cushion ua kom muaj qhov ntim ntau dua ntawm cov qauv ua tiav.
Dab tsi zoo sib xws ntawm Sucrose Gradient thiab Sucrose Cushion Ultracentrifugation?
- Sucrose gradient thiab sucrose cushion ultracentrifugation yog ob txoj kev siv los cais cov sib xyaw ntawm macromolecules.
- Nyob rau hauv ob txoj kev, siv sucrose gradient.
- Centrifugation yog ib kauj ruam ntawm ob txoj hauv kev.
- Cov molecules sediment ua ib pawg lossis ib cheeb tsam hauv txhua txoj kev.
Qhov txawv ntawm Sucrose Gradient thiab Sucrose Cushion Ultracentrifugation yog dab tsi?
Sucrose gradient ultracentrifugation yog cov txheej txheem uas siv cov sucrose gradient txuas ntxiv hauv cov raj centrifuge thaum sucrose cushion ultracentrifugation yog cov txheej txheem uas siv cov sucrose gradient tsis tu ncua. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm sucrose gradient thiab sucrose cushion ultracentrifugation.
Ntxiv mus, sucrose cushion ultracentrifugation tso cai kom ntim ntau dua ntawm cov qauv ua tiav thaum sucrose gradient ultracentrifugation tso cai rau qhov sib piv qis ntawm cov qauv ua tiav.
Hauv qab infographic tabulates ntau qhov sib txawv ntawm sucrose gradient thiab sucrose cushion ultracentrifugation.
Summary – Sucrose Gradient vs Sucrose Cushion Ultracentrifugation
Sucrose gradient ultracentrifugation cais macromolecules siv cov sucrose gradient txuas ntxiv. Nyob rau hauv sib piv, sucrose cushion ultracentrifugation siv ib tug discontinuous sucrose gradient nyob rau hauv thiaj li yuav cais tej yam hais nyob rau hauv ib tug sib tov. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm sucrose gradient thiab sucrose cushion ultracentrifugation. Txoj kev sucrose cushion tso cai rau kev sau ntawm morphologically intact particles vim nws tsis ua rau txhua yam kev nyuaj siab, tsis zoo li sucrose gradient ultracentrifugation, uas crushes molecules rau hauv qab ntawm lub raj. Ntxiv mus, sucrose cushion ultracentrifugation tso cai rau lub ntim ntau dua ntawm cov qauv ua tiav dua li sucrose gradient ultracentrifugation.
