Qhov sib txawv tseem ceeb ntawm hierarchical thiab tag nrho genome shotgun sequencing yog tias nyob rau hauv hierarchical shotgun sequencing, lub genome yog tawg mus rau hauv loj fragments ua ntej sequencing thaum, tag nrho cov genome shotgun sequencing, tag nrho cov genome yog tawg mus rau hauv me me fragments rau sequencing..
Sequencing yog cov txheej txheem tseem ceeb hauv cov txheej txheem molecular, tshwj xeeb tshaj yog nyob rau hauv kev txheeb xyuas qhov tseeb ntawm hom. Qhov tseeb, nws yog cov txheej txheem ntawm kev txheeb xyuas qhov tseeb ntawm nucleotides hauv cov noob lossis cov genome. Muaj ntau ntau cov txheej txheem rau sequence. Qee qhov no suav nrog Maxam Gilbert Method, Sanger Method thiab automated Sanger method.
Hierarchical Shotgun Sequencing yog dab tsi?
Hierarchical Shotgun sequencing yog ib txoj hauv kev ntawm kev sib tw. Nws tseem hu ua 'sab saum toj-down sequencing'. Ntxiv mus, txoj kev no muaj ob kauj ruam: thawj kauj ruam yog genome amplification, thiab theem ob yog genome fragmentation. Nyob rau hauv txoj kev no, lub genome yog sheared mus rau hauv loj fragments ua ntej. Nws yog ua raws li los ntawm cloning ntawm cov khoom loj no rau hauv ntau lub tswv yim siv vectors lossis cov chromosomes dag. Tom qab ntawd, cov vectors recombinant tau muab tso rau hauv ib lub tsev qiv ntawv. Thaum lub sij hawm txhaj koob tshuaj tivthaiv, cov recombinant vector clones undergo sequencing tus kheej. Cov fragments nyob rau hauv lub clones raug txwv kev zom kom ntxiv txo qhov luaj li cas ntawm genome, yog li mus ua sequencing.
Daim duab 01: Hierarchical Shotgun Sequencing
Thaum lub sijhawm tua phom hierarchical sequencing, cov chunks loj ntawm fragments yuav tsum tau teem nyob rau hauv kev txiav txim, ua ntej tua phom sequencing. Nws tshwm sim nrog kev pab los ntawm cov cim molecular uas tau muab tso rau thaum lub sijhawm cloning.
Hierarchical shotgun sequencing tsim ib daim ntawv qhia noob qes qis. Tab sis nws tsim ib daim ntawv qhia ua ntu zus. Yog li, nws yuav siv sij hawm ntev dua li kev sib txuas ncaj qha. Tag nrho cov txheej txheem qeeb vim yog tsim cov tsev qiv ntawv vector rov ua dua. Puas yog nws tseem yog cov txheej txheem ua haujlwm hnyav. Txawm li cas los xij, tam sim no, cov txheej txheem yog automated los ua haujlwm yooj yim.
Dab tsi yog Tag Nrho Genome Shotgun Sequencing?
Tag nrho cov genome phom sib txuas yog ib kauj ruam ua ntu zus. Hauv cov txheej txheem no, tag nrho cov genome shearing tshwm sim thawj zaug. Tom qab qhov no, txhua qhov me me ntawm cov khoom no tau ua raws li qhov sib txawv. Tom qab ua tiav cov sequences, cov sequence tsom xam yuav tshwm sim. Thaum lub sij hawm soj ntsuam ib ntus, qhov sib tshooj sib tshooj raug tshem tawm, thiab kev soj ntsuam tsis yog txheej txheem xaj. Yog li ntawd, kev sib sau ua ke ntawm cov sequences yog cov txheej txheem tsawg dua hauv kev sib piv rau hierarchical shotgun sequencing. Txawm li cas los xij, cov txheej txheem ntawm tag nrho genome shotgun sequencing yog sai dua, thiab kev soj ntsuam ntawm tag nrho cov genome tuaj yeem tshwm sim ntawm ib qho piv txwv.
Dab tsi yog qhov zoo sib xws ntawm Hierarchical thiab tag nrho Genome Shotgun Sequencing?
- Hierarchical thiab tag nrho genome shotgun sequencing yog ob txoj hauv kev ua ke.
- Ob leeg tau txais Sanger sequencing lossis automated Sanger sequencing txoj kev.
- Lawv koom nrog kev sib txuas DNA thiab RNA fragments.
- Ntxiv mus, ob txoj hauv kev tseem ceeb hauv kev kuaj mob molecular thiab tshawb fawb lub hom phiaj.
- Tam sim no, ob qho tib si cov txheej txheem yog automated lossis computer-based.
- Ob txoj kev vam khom ntawm kev tawg ntawm genome.
Dab tsi yog qhov txawv ntawm Hierarchical thiab tag nrho Genome Shotgun Sequencing?
Hierarchical shotgun sequencing generates loj me me fragments nyob rau hauv sib piv rau tag nrho-genome shotgun sequencing, uas ua hauj lwm nrog me me fragments ntawm sequences. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm hierarchical thiab tag nrho genome shotgun sequencing. Tsis tas li ntawd, lub hierarchical shotgun sequencing muaj ob kauj ruam loj, thaum tag nrho-genome shotgun sequencing cia siab rau ib kauj ruam loj.
Tsis tas li ntawd, lwm qhov sib txawv ntawm hierarchical thiab tag nrho genome shotgun sequencing yog qhov kev daws teeb meem siab dua hauv tag nrho cov genome shotgun sequencing piv rau hierarchical shotgun sequencing.
Cov hauv qab no infographic qhia txog qhov sib txawv ntawm hierarchical thiab tag nrho cov genome shotgun sequencing.
Summary – Hierarchical vs Whole Genome Shotgun Sequencing
Hierarchical thiab tag nrho-genome phom sib luag yog ob txoj hauv kev los ua kom cov genomes loj. Cov phom tua phom hierarchical yog ob-kauj ruam ntawm kev sib txuas uas cov genome tau tawg mus rau hauv cov khoom loj dua. Nyob rau hauv sib piv, tag nrho-genome shotgun sequencing yog ib kauj ruam sequencing qhov twg cov genome yog tawg mus rau hauv me me fragments thiab ncaj qha sequenced. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm hierarchical thiab tag nrho genome shotgun sequencing. Tsis tas li ntawd, cov txheej txheem yuav txawv ntawm ob txoj kev.
