Key Difference – 16s rRNA vs 16s rDNA
Ribosomes yog qhov chaw lom neeg ntawm cov protein synthesis hauv txhua yam kab mob. Ribosomes muaj ob yam khoom; me subunit thiab ib tug loj subunit. Cov kab mob prokaryotic thiab Eukaryotic kab mob sib txawv ntawm cov ribosome uas lawv muaj. Txhua lub subunit yog tsim los ntawm ribosomal RNA thiab cov protein sib txawv. Ob lub subunits no haum ua ke thiab ua haujlwm ua ib qho thaum lub sij hawm tsim cov protein. Prokaryotic ribosomes yog 70S thiab lawv muaj li ntawm 30S me subunit thiab 50S loj subunit. Eukaryotic ribosomes yog 80S thiab lawv muaj li ntawm 40S me subunit thiab 60S loj subunit. Hauv prokaryotes, ribosomal RNA ntawm me me subunit ntawm ribosomes hu ua 16s rRNA. Qhov 16s rRNA no tau sau los ntawm chromosomal DNA uas yog hu ua 16s rDNA. 16s rDNA yog cov noob uas tsim 16s rRNA los ntawm kev sau ntawv. Qhov sib txawv tseem ceeb ntawm 16s rRNA thiab 16s rDNA yog tias 16s rRNA yog cov ntawv sau ib leeg-stranded ribosomal RNA uas yog ib feem ntawm cov subunit me me ntawm prokaryotes thaum 16s rDNA yog ob-stranded chromosomal DNA lossis cov noob uas codeR 16s.. Gene ntawm 16s rRNA yog 16s rDNA.
16s rRNA yog dab tsi?
rRNA yog ib feem ntawm ribosomes. 16s rRNA yog qhov tshwj xeeb ntawm 30S me subunit ntawm prokaryotic ribosome uas khi nrog Shine-Dalgarno ib ntus. Qhov 16s rRNA ntu no qhia tau hais tias muaj kev sib txawv ntawm cov kab mob. Li no, nws tuaj yeem siv rau cov kab mob phylogeny thiab taxonomy.
16s rDNA yog dab tsi?
Prokaryotes muaj 70S ribosomes. Lub subunit me me ntawm prokaryotic ribosomes yog 30S. Lub ribosomal RNA (rRNA) ntawm 30S me subunit yog hu ua 16s rRNA, thiab cov noob 16s rDNA codes nws. Li no 16s rDNA hu ua 16s rRNA noob. 16s rDNA yog chromosomal DNA. Nws yog ob-stranded, thiab nws yog ib tug gene tsim ntawm coding thiab noncoding cheeb tsam. Thaum 16s rDNA noob tau sau tseg, nws tsim 16s rRNA ib ntus. 16s rDNA yog universal DNA sequence hauv prokaryotes. Txawm li cas los xij, qhov sib lawv liag ntawm 16s rDNA ntawm cov prokaryotes sib txawv. Nws pab txhawb kev siv 16s rDNA ib ntus hauv kev txheeb xyuas cov kab mob kom raug thiab tseem rau kev tshawb pom cov kab mob tshiab.
16s rDNA ua lub luag haujlwm tseem ceeb hauv cov kab mob phylogeny thiab taxonomy. Yog li ntawd, nws yog siv raws li ib tug txhim khu kev qha molecular marker nyob rau hauv phylogenetic kev tshawb fawb ntawm prokaryotes vim nws yog heev conserved ntawm ntau hom. 16s rDNA nucleotide ib ntus muaj cuaj qhov chaw sib txawv (V1-V9) uas muab qhov zoo rau kev sib txawv ntawm cov kab mob thiab archaea.
Daim duab 01: DNA thiab RNA
Kev sib luag ntawm 16s rDNA noob tau ua kom yooj yim rau kev faib cov kab mob mus rau hauv hom tshiab lossis genera. Yog li ntawd, cov noob no yog siv nyob rau hauv cov chaw soj nstuam molecular ua tus cim kev saib xyuas hauv tsev ntau tshaj plaws rau kev txheeb xyuas cov kab mob microbes. Muaj ntau qhov laj thawj uas ua rau 16srDNA yog qhov cim zoo tshaj plaws rau kev txheeb xyuas cov kab mob xws li muaj 16srDNA hauv txhua tus kab mob, qhov tsis hloov pauv ntawm kev ua haujlwm ntawm 16s rDNA noob nyob rau lub sijhawm, thiab qhov loj ntawm 16s rDNA uas ua rau nws. txaus rau cov ntaub ntawv lub hom phiaj.
Dab tsi yog qhov zoo sib xws ntawm 16s rRNA thiab 16s rDNA?
- ob leeg yog nucleic acids.
- Ob leeg yog tsim los ntawm nucleotides.
- Ob leeg muaj feem cuam tshuam rau ribosomal RNA.
Qhov txawv ntawm 16s rRNA thiab 16s rDNA yog dab tsi?
16s rRNA vs 16s rDNA |
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| 16s rRNA yog ribosomal RNA tivthaiv ntawm lub subunit me me ntawm 30s ribosome ntawm prokaryotes. | 16s rDNA yog chromosomal DNA uas encodes rau 16s rRNA ib ntus ntawm prokaryotes. |
| 16s rRNA yog ib leeg-stranded. | 16s rDNA yog ob txoj hlua |
| Gene or Sequence | |
| 16s rRNA yog ib qho RNA ntawm cov noob. | 16s rDNA is a gene. |
| Coding Sequence | |
| 16s rRNA tsuas muaj qhov coding sequence xwb. | 16s rDNA muaj ob qho tib si coding thiab tsis-coding strands. |
| Uracil Base | |
| 16s rRNA muaj Uracil hauv paus hauv nws cov nucleotide ib ntus. | 16s rDNA tsis muaj lub hauv paus Uracil hauv nws cov nucleotide sequences. |
| Thymin Base | |
| 16s rRNA tsis muaj Thymine hauv paus hauv nws cov nucleotide ib ntus. | 16s rDNA muaj Thymine hauv paus hauv nws cov nucleotide sequences. |
| Synthesis | |
| 16s rRNA yog tsim los ntawm kev hloov pauv ntawm 16s rDNA gene. | 16s rDNA yog nyob rau hauv genome ntawm cov prokaryotes. |
Summary – 16s rRNA vs 16s rDNA
16s rRNA yog ribosomal RNA tivthaiv ntawm cov subunit me me ntawm ribosomes ntawm prokaryotes. Lub gene 16s rDNA encodes no RNA ib ntus. 16s rRNA yog ib leeg-stranded thiab 16s rDNA yog ob-stranded. Qhov no yog qhov txawv ntawm 16s rRNA thiab 16s rDNA.
Download the PDF 16s rRNA vs 16s rDNA
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline lub hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm 16s rRNA thiab 16s rDNA
