Key Difference – Ligase vs Lyase
Qhov sib txawv tseem ceeb ntawm ligase thiab lyase yog tias ligases koom nrog hauv kev tsim cov tshuaj bonds whereas lyases ua rau tawg ntawm cov tshuaj bonds.
Ligases thiab lyases yog cov enzymes. Enzymes yog macromolecular compounds uas tuaj yeem ua cov tshuaj lom neeg lom neeg. Cov tebchaw no tuaj yeem ua rau nce cov tshuaj tiv thaiv ntawm qee cov tshuaj tiv thaiv los ntawm kev txo qhov ua kom lub zog thaiv. Ligases yog ib hom enzymes uas coj txog ligation ntawm DNA los yog lwm yam khoom. Yog li ntawd, ligases ua rau kev sib txuas ntawm cov khoom sib txawv. Lyases yog cov enzymes uas ua rau tawg ntawm cov tshuaj sib txuas uas tsis yog hydrolysis thiab oxidation.
Ligase yog dab tsi?
Ligase yog ib qho enzyme uas coj txog ligation ntawm DNA lossis lwm yam khoom. Qhov no txhais tau tias ligases catalyzes kev koom ua ke ntawm ob yam khoom. Qhov kev koom ua ke ntawm ob yam khoom no ua rau muaj kev tsim cov tshuaj lom neeg tshiab. Ligases tseem hu ua synthetases vim tias cov enzymes no ua rau tsim cov tshuaj tshiab.
Ligases ua rau tsim cov molecules loj dua li cov khoom pib. Hydrolysis tshwm sim thaum lub sijhawm ua haujlwm ntawm ligase. Muaj txog 50 ligases sib txawv. Cov ligases no xav tau lub zog muab los ntawm ATP (Adenosine triphosphate). Thaum lub ligase tau siv lub zog los ntawm ATP, ATP hloov mus rau ADP (Adenosine diphosphate).
Daim duab 01: Kev Ua ntawm DNA Ligase
Ib qho piv txwv ntawm chav kawm no yog DNA ligase. Cov enzyme no yog siv los npog cov qhov qhib tam sim no hauv DNA strand. DNA ligases tuaj yeem koom nrog kev sib txuas ntxiv thaum lub sijhawm DNA rov ua dua. Vim li no, ligases tseem hu ua kua nplaum molecular.
Lyase yog dab tsi?
Lyases yog ib pab pawg ntawm cov enzymes uas ua rau muaj kev tawg ntawm ntau yam tshuaj sib txuas los ntawm kev txhais tau tias tsis yog hydrolysis thiab oxidation, feem ntau tsim ib daim ntawv cog lus tshiab lossis lub nplhaib tshiab. Qhov no txhais tau tias lyases catalyze lub cleavage ntawm tej yam tshuaj bonds.
Cov kev sib cog lus no tshwm sim nyob rau hauv daim ntawv ntawm kev tshem tawm uas ua rau cov khoom tsis txaus ntseeg (ib qho chaw uas muaj ob daim ntawv cog lus). Txawm li cas los xij, hydrolysis lossis oxidation tsis tshwm sim hauv qhov catalysis. Lyases ua rau ib qho reactant muab ob yam khoom sib txawv; ib yam khoom yog cov khoom tsis sib xyaw, thiab lwm yam khoom yog cov khoom tshem tawm.
Qee cov tshuaj tiv thaiv catalyzed los ntawm lyases yog thim rov qab thaum feem ntau lwm cov tshuaj tiv thaiv tsis tuaj yeem thim rov qab. Tab sis rau qhov rov qab tshwm sim tshwm sim, lyases xav tau ob reactants; unsaturated compound thiab cov molecules me me uas yuav hloov mus rau ob daim ntawv cog lus.
Daim duab 02: Ib qho piv txwv ntawm Lyase Action hauv Glycolysis
Ntau yam kev cuam tshuam hauv glycolysis thiab Krebs voj voog hauv peb lub cev yog catalyzed los ntawm lyases. Piv txwv li: Cleavage ntawm fructose 1, 6-bisphosphate (F 1, 6-BP) tsim ob lub tebchaw hu ua glyceraldehyde-3-phosphate (GAP) thiab dihydroxyacetone phosphate (DHAP). Cov tshuaj tiv thaiv no tau muab rau hauv daim duab saum toj no.
Qhov txawv ntawm Ligase thiab Lyase yog dab tsi?
Ligase vs Lyase |
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| Ligase yog ib qho enzyme uas coj txog ligation ntawm DNA lossis lwm yam khoom. | Lyases yog ib pab pawg ntawm cov enzymes uas ua rau kev tawg ntawm ntau yam tshuaj tiv thaiv los ntawm kev txhais tau tias tsis yog hydrolysis thiab oxidation, feem ntau tsim ib daim ntawv cog lus tshiab lossis lub nplhaib tshiab. |
| Tshuaj Bonds | |
| Ligases ua rau tsim daim ntawv cog lus. | Lyases ua rau daim ntawv cog lus cleavages. |
| Reaction Type | |
| Ligases ua los ntawm cov tshuaj tiv thaiv hydrolysis. | Lyases ua los ntawm kev tshem tawm cov tshuaj tiv thaiv. |
| Reactants | |
| Ligases ua rau ob lub reactants ib zaug. | Lyases ua rau ib qho reactant ib zaug. |
Summary – Ligase vs Lyase
Lyases thiab ligases yog hom enzymes. Cov no feem ntau pom nyob rau hauv cov kab mob lom raws li cov khoom siv roj ntsha. Qhov sib txawv ntawm ligase thiab lyase yog tias ligases rhuav tshem cov tshuaj lom neeg thaum lyases koom nrog hauv kev tsim cov tshuaj lom neeg.
