Qhov sib txawv tseem ceeb ntawm Anthrone thiab Molisch xeem yog qhov kev sim Anthrone tuaj yeem siv los kuaj xyuas cov carbohydrates siv cov xim xiav-ntsuab, qhov kev kuaj Molisch tuaj yeem siv los kuaj xyuas cov carbohydrates siv cov xim violet.
Anthrone kuaj yog qhov ntsuas ceev thiab yooj yim rau kev ntsuas cov carbohydrates uas tshwm sim tsis pub dawb lossis khi rau ib qho lipids lossis cov protein. Molisch xeem, ntawm qhov tod tes, yog ib qho kev ntsuam xyuas uas muaj txiaj ntsig zoo hauv kev kuaj pom muaj cov carbohydrates nyob ntawm lub cev qhuav dej ntawm cov carbohydrate los ntawm sulfuric acid hauv qhov ntsuas reagent.
Anthrone Test yog dab tsi?
Anthrone test yog ib qho kev ntsuas ntsuas rau cov carbohydrates. Nws yog ib txoj hauv kev sai thiab yooj yim rau kev txheeb xyuas cov carbohydrates uas tshwm sim tsis pub dawb lossis khi rau ib qho lipids lossis cov protein. Qhov kev sim no muaj ob lub hom phiaj tseem ceeb: txhawm rau txheeb xyuas qhov muaj cov carbohydrates hauv cov tshuaj uas tau muab thiab ntsuas qhov concentration ntawm cov carbohydrates dawb thiab khi rau hauv kev daws teeb meem.
Daim duab 01: Anthrone Test Result
Raws li qhov kev sim no, yog tias muaj cov carbohydrate nyob rau hauv daim ntawv ntawm cov carbohydrate dawb li polysaccharides, monosaccharides, los yog khi li hauv glycoprotein lossis glycolipid, cov carbohydrate yuav raug kuaj pom los ntawm cov concentrated acid hauv Anthrone reagent. Nws yuav ua ntej hydrolyze cov carbohydrate rau hauv cov khoom monosaccharides. Tom qab ntawd cov concentrated acid catalyzes lub cev qhuav dej ntawm monosaccharides tsim furfural los yog hydroxyl furfural.
Tom qab ntawd, cov tsim furfural lossis hydroxyl furfural condenses nrog ob lub molecules ntawm naphthol los ntawm Anthrone reagent kom muab lub xiav-ntsuab complex. Raws li cov kauj ruam kawg, peb tuaj yeem txheeb xyuas qhov nyuaj tsim los ntawm kev ntsuas qhov nqus tau siv lub spectrophotometer nyob rau hauv 620 nm wavelength los yog hauv cov xim liab lim colorimeter.
Lub Anthrone reagent siv hauv qhov kev sim no yog tsim los ntawm kev sib cais 2 grams ntawm Anthrone hauv ib liter ntawm cov concentrated sulfuric acid. Peb yuav tsum tau siv cov tshuaj reagent tshiab tshiab rau qhov kev ntsuam xyuas no.
Molisch Test yog dab tsi?
Molisch xeem yog ib qho kev ntsuam xyuas uas muaj txiaj ntsig zoo hauv kev kuaj pom muaj cov carbohydrates nyob ntawm seb lub cev qhuav dej ntawm cov carbohydrate los ntawm sulfuric acid hauv qhov ntsuas reagent. Qee zaum, hydrochloric acid muaj nyob rau hauv reagent es tsis txhob sulfuric acid. Nyob ntawd, cov kua qaub tuaj yeem dehydrate cov carbohydrate uas ua rau aldehyde. Qhov no aldehyde ces condenses nrog ob molecules ntawm phenol thiab muab ib lub nplhaib violet.
Daim duab 02: Molisch Test
Qhov kev sim no tau muab npe tom qab Hans Molisch, Austrian botanist. Hauv cov txheej txheem, cov tshuaj ntsuam xyuas yuav tsum tau ua ke nrog me me ntawm Molisch's reagent siv lub raj kuaj. Tom qab qhov sib tov no, peb yuav tsum ntxiv me me ntawm sulfuric acid rau ntawm ob sab ntawm lub raj kuaj, uas yuav tsum tau muab tso rau ntawm lub kaum sab xis thaum lub sijhawm ntxiv no. Qhov no tsim ib txheej violet los qhia tias muaj cov carbohydrate.
Qhov txawv ntawm Anthrone thiab Molisch Test yog dab tsi?
Anthrone test thiab Molisch test yog qhov tseem ceeb hauv kev kuaj pom cov carbohydrates hauv cov qauv kuaj. Qhov sib txawv tseem ceeb ntawm Anthrone thiab Molisch xeem yog qhov kev sim Anthrone tuaj yeem siv los kuaj xyuas cov carbohydrates uas siv cov xim xiav-ntsuab, qhov kev kuaj Molisch tuaj yeem siv los kuaj xyuas cov carbohydrates siv cov xim violet.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm Anthrone thiab Molisch xeem hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – Anthrone vs Molisch Test
Anthrone test thiab Molisch test yog ob hom kev ntsuas ntsuas. Qhov sib txawv tseem ceeb ntawm Anthrone thiab Molisch xeem yog qhov kev sim Anthrone tuaj yeem siv los kuaj xyuas cov carbohydrates siv cov xim xiav-ntsuab, qhov kev kuaj Molisch tuaj yeem siv los txheeb xyuas cov carbohydrates siv cov xim violet.
