Qhov sib txawv tseem ceeb ntawm pBR322 thiab pUC19 yog pBR322 yog plasmid vector uas yog 4361 puag khub ntev, thaum pUC19 yog plasmid vector uas yog 2686 lub hauv paus khub ntev.
Ib cloning vector yog ib qho me me ntawm DNA uas tuaj yeem khaws cia hauv lub cev ruaj khov. Nws kuj yog ib qho me me ntawm DNA rau hauv uas cov DNA txawv teb chaws tuaj yeem muab tso rau lub hom phiaj cloning. Ib lub vector muaj cov yam ntxwv tseem ceeb uas tso cai rau kev yooj yim nkag ntawm cov DNA txawv teb chaws rau hauv vectors, xws li cov chaw txwv, cov cim xaiv tau, cov neeg sau xov xwm, thiab cov ntsiab lus ntawm kev qhia. Cloning tau ua thawj zaug siv E.coli ua. Cloning vectors rau E. coli muaj xws li plasmids, bacteriophages, cosmids, thiab cov kab mob sib xyaw ua ke (BAC). Feem ntau siv vectors yog genetically engineered plasmids. pBR322 thiab pUC19 yog ob hom plasmid vectors.
YPBR322 yog dab tsi?
pBR322 yog plasmid vector. Nws yog ib qho ntawm thawj zaug siv cloning vectors rau E. coli. Cov vector no tau tsim thawj zaug hauv xyoo 1977 hauv chav kuaj ntawm Herbert Boyer ntawm University of California, San Francisco. Nws yog ib puag ncig ob-stranded DNA uas yog 4361 puag khub ntev.
Daim duab 01: pBR322
No plasmid vector muaj qhov tshwj xeeb. Nquag, nws muaj ob hom tshuaj tiv thaiv kab mob: gene bla encoding lub ampicillin resistance (AmpR) protein thiab gene tetA encoding the tetracycline resistance (TetR) cov protein. Ntxiv mus, nws muaj keeb kwm ntawm kev rov ua dua ntawm pMB1. Lub plasmid vector no kuj muaj cov noob caj noob ces uas encodes tus txwv ntawm tus lej plasmid. Tsis tas li ntawd, cov vector no muaj qhov tshwj xeeb txwv qhov chaw rau ntau tshaj 40 txwv enzymes. Kaum ib ntawm plaub caug qhov chaw txwv tsis pub nyob hauv TetR gene. Muaj ob qhov chaw txwv rau kev txwv enzymes HindIII thiab ClaI nyob rau hauv kev txhawb nqa ntawm Tet R noob. Tsis tas li ntawd, muaj rau qhov chaw txwv tseem ceeb heev nyob rau hauv AmpR noob. pBR322 yog cov ntawv luam tsawg tus lej cloning vector. Nws kwv yees li muab 20 daim ntawv plasmid ib kab kab mob. Qhov hnyav molecular ntawm vector yog 2.83 × 10 6 d altons.
pUC19 yog dab tsi?
pUC19 yog plasmid vector uas yog 2686 lub hauv paus khub ntev. Nws yog ib qho ntawm cov plasmid vectors tsim los ntawm Joachim Messing thiab cov neeg ua haujlwm. Kev ua haujlwm ntxov ntawm cov plasmid series no tau ua nyob rau hauv University of California. Nws yog ib puag ncig ob-stranded DNA.
Daim duab 02: pUC19
No plasmid vector kuj muaj cov yam ntxwv tseem ceeb. Nws muaj N terminal fragment ntawm β glactosidase (lacz) noob. Nws kuj muaj ntau qhov chaw cloning (MCS) cheeb tsam. Cov cheeb tsam no tau muab faib ua codons 6 thiab 7 ntawm lacz gene. MCS cheeb tsam muab ntau qhov kev txwv endonucleases txwv qhov chaw. Ntxiv nrog rau β glactosidase noob, pUC19 kuj tseem encodes rau ampicillin kuj gene (AmpR). Ntxiv mus, lub hauv paus chiv keeb ntawm replication yog muab los ntawm plasmid pMB1. Txawm hais tias pUC19 yog qhov me me, nws muaj tus lej luam siab (500-700 daim ntawv luam rau ib lub xov tooj ntawm tes). Qhov hnyav molecular ntawm no plasmid vector yog 1.75 × 10 6 d altons.
Dab tsi yog qhov zoo sib xws ntawm pBR322 thiab pUC19?
- pBR322 thiab pUC19 yog ob hom plasmid vectors.
- Lawv yog cov neeg nyiam cloning vectors rau coli.
- Ob lub plasmid vectors muaj tib lub hauv paus chiv keeb ntawm daim ntawv luam ntawm plasmid pMB1.
- Cov vectors no muaj ntau qhov chaw txwv.
- Ob lub plasmid vectors muaj cov tshuaj ampicillin tsis kam ua tus cim xaiv.
Qhov txawv ntawm pBR322 thiab pUC19 yog dab tsi?
pBR322 yog plasmid vector uas yog 4361 puag khub hauv qhov ntev, thaum pUC19 yog plasmid vector uas yog 2686 lub hauv paus khub ntev. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm pBR322 thiab pUC19. Tsis tas li ntawd, pBR322 yog tus lej luam qis plasmid vector, thaum pUC19 yog tus lej luam siab plasmid vector.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm pBR322 thiab pUC19 hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – pBR322 vs pUC19
Qhov feem ntau siv cloning vectors rau E.coli yog genetic engineered plasmids.pBR322 thiab pUC19 yog ob hom plasmid vectors. pBR322 yog ib tug plasmid vector uas yog 4361 puag khub nyob rau hauv ntev, thaum pUC19 yog ib tug plasmid vector uas yog 2686 puag khub nyob rau hauv ntev. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm pBR322 thiab pUC19.
