Qhov sib txawv tseem ceeb ntawm theem rov qab thiab theem ib txwm HPLC yog qhov rov qab theem HPLC siv lub sijhawm tsis muaj polar nyob ruaj khov thiab lub polar mobile theem thaum lub sijhawm ib txwm HPLC siv lub polar nyob ruaj ruaj thiab qis dua polar mobile theem.
Hnub theem HPLC yog cov txheej txheem qub tshaj plaws ntawm HPLC uas Tswett siv rau hauv nws cov kev sib cais ntawm cov nroj tsuag rho tawm; nws siv chalk nyob rau hauv ib kem iav. Qhov no yog hom classical ntawm chromatography uas coj mus rau naming nws li "ib txwm" txheej txheem. Rov qab-theem HPLC, ntawm qhov tod tes, yog qhov sib txawv ntawm cov txheej txheem ib txwm, uas cov kws tshawb fawb tau tsim tsis ntev los no.
HP rov qab Phase HPLC yog dab tsi?
Rov qab theem HPLC yog cov txheej txheem chromatographic uas peb siv theem hydrophobic nyob ruaj khov. Ntawm txhua txoj hauv kev HPLC, peb siv txoj kev no rau kwv yees li 70% vim tias nws siv dav dav, thiab rov tsim dua tshiab. Theem nyob ruaj ruaj yog nonpolar thiab lub sijhawm txawb yog polar.
Feem ntau, cov kws tshawb fawb siv cov dej sib xyaw ua ke nrog cov miscible, polar organic solvent, xws li acetonitrile lossis methanol ua lub sijhawm txawb. Yog li ntawd, cov analytes cuam tshuam nrog lub nonpolar nyob ruaj ruaj theem.
Pib HPLC yog dab tsi?
Ib txwm theem HPLC yog cov txheej txheem chromatographic uas peb siv theem hydrophilic nyob ruaj khov. Nws yog ib txwm siv ntawm HPLC, txawm tias peb tsis siv ntau npaum li ntawd. Cov theem nyob ruaj ruaj yog polar, thiab theem mobile tsis yog polar. Qhov tseem ceeb tshaj, lub sijhawm txawb ntawm cov txheej txheem no yog 100% organic. Nws txhais tau tias tsis muaj dej siv rau qhov no.
Daim duab 01: Ib qho piv txwv profile rau theem ib txwm thiab rov qab theem HPLC chromatograms raws li polarity ntawm cov khoom hauv qhov ntsuas.
Feem ntau, cov theem nyob ruaj ruaj muaj silica, cyano, diol, amino bonded theem, thiab lwm yam. Cov theem txawb muaj xws li cov kuab tshuaj organic xws li hexane, ethyl acetate, thiab lwm yam. Cov txheej txheem no yog los ntawm kev khaws cov kuab tshuaj los yog tshuaj ntsuam xyuas molecules mus rau lub polar nyob ruaj ruaj theem.
Dab tsi yog qhov txawv ntawm Cov Phaj Rov Qab thiab Qib HPLC?
Rov qab theem HPLC yog cov txheej txheem chromatographic uas peb siv theem hydrophobic nyob ruaj khov. Cov theem nyob ruaj khov ntawm cov txheej txheem no tsis yog polar thaum lub sijhawm txawb yog polar. Ntxiv mus, cov kws tshawb fawb siv cov dej sib xyaw ua ke nrog cov miscible, polar organic solvent, xws li acetonitrile lossis methanol raws li lub xov tooj ntawm tes hauv theem HPLC. Hauv qhov sib piv, cov theem ib txwm HPLC yog cov txheej txheem chromatographic uas peb siv cov theem hydrophilic nyob ruaj ruaj. Lub sijhawm nyob ruaj khov ntawm cov txheej txheem no yog polar thaum lub sijhawm txawb tsis yog polar. Ntxiv rau qhov ntawd, cov kws tshawb fawb siv cov kuab tshuaj organic xws li hexane, ethyl acetate, thiab lwm yam raws li cov theem ntawm lub cev ntawm HPLC. Cov nram qab no infographic nthuav tawm qhov sib txawv ntawm theem rov qab thiab theem ib txwm HPLC hauv daim ntawv teev npe.
Summary – Rov qab Theem vs Qib HPLC
Rov qab theem thiab theem ib txwm HPLC cov txheej txheem yog ob txoj kev ua kua chromatographic. Qhov sib txawv tseem ceeb ntawm theem rov qab thiab theem ib txwm HPLC yog qhov rov qab theem HPLC siv lub sijhawm tsis muaj polar nyob ruaj khov thiab lub hnub qub txawb thaum lub sijhawm HPLC siv lub polar nyob ruaj khov thiab qis dua polar mobile theem.
