Qhov sib txawv tseem ceeb ntawm Klenow fragment thiab DNA polymerase 1 yog tias Klenow fragment yog ib feem ntawm DNA polymerase 1 uas tsis muaj 5′ txog 3′ exonuclease kev ua haujlwm thaum DNA polymerase yog ib qho enzyme ntawm E. coli uas muaj tag nrho peb. domains suav nrog 5′ txog 3′ kev ua haujlwm exonuclease.
DNA polymerase yog ib qho ntawm cov enzymes tseem ceeb koom nrog DNA replication hauv cov kab mob nyob. Muaj ntau hom DNA polymerase enzyme pom nyob rau hauv eukaryotes thiab prokaryotes raws li qhov loj thiab cov duab. DNA polymerase 1, 2 thiab 3 tsuas yog pom nyob rau hauv cov kab mob prokaryotic, thiab lawv ua lub luag haujlwm sib txawv hauv DNA replication. DNA polymerase 1 muaj peb qhov tseem ceeb nrog 5' rau 3' polymerase, 3' rau 5' exonuclease kev ua thiab 5' rau 3' exonuclease kev ua. Klenow fragment yog cov khoom proteolytic ntawm DNA polymerase 1. Nws muaj 5 mus rau 3′ polymerase thiab 3′ rau 5′ exonuclease cov dej num ntawm cov DNA polymerase 1, tab sis nws tsis muaj 5′ rau 3′ exonuclease kev ua.
Klenow Fragment yog dab tsi?
Klenow fragment yog ib feem loj ntawm DNA polymerase 1. Tsis zoo li DNA polymerase 1, 5′ txog 3′ exonuclease kev ua tsis pom hauv Klenow fragment vim nws tsis muaj 5′ rau 3′ rau pem hauv ntej exonuclease domain. Klenow fragments yog tsim los ntawm digesting DNA polymerase 1 nrog ib tug protease hu ua subtilisin, uas yog ib tug protein-digesting enzyme. Hauv qee qhov kev siv, 3′ txog 5′ exonuclease kev ua ntawm Klenow fragment ua tsis tau zoo. Nws tuaj yeem raug tshem tawm los ntawm kev qhia txog kev hloov pauv hauv cov noob uas encodes Klenow fragment. Qhov tshwm sim enzyme uas tsuas muaj 5' rau 3' polymerase kev ua haujlwm yog hu ua exo-Klenow fragment. Morphologically, Klenow fragment zoo li tib neeg sab tes xis. Nws muaj N-terminal 3′-5′ exonuclease domain thiab C-terminal polymerase domain sib cais los ntawm 30 A.
Daim duab 01: Klenow Fragment thiab DNA Polymerase 1
Klenow fragment yog ib qho cuab yeej tseem ceeb hauv cov txheej txheem lom neeg molecular. Klenow fragments muaj ntau yam siv. Lawv yog cov tseem ceeb heev nyob rau hauv blunt-kawg cloning thiaj li yuav tsim blunt fragments. Klenow fragments tuaj yeem tshem tawm 3′ overhangs lossis sau 5′ overhangs rau daim ntawv blunt kawg. Ntxiv mus, Klenow fragments yog siv los ua ke ob-stranded DNA los ntawm ib tug strands. Tsis tas li ntawd, lawv tau siv los npaj cov tshuaj tiv thaiv hluav taws xob uas siv cov 5'to 3' DNA dependent DNA polymerase kev ua haujlwm.
DNA Polymerase 1 yog dab tsi?
DNA polymerase 1 (Pol 1) yog ib qho enzyme pom hauv prokaryotes uas xav tau rau cov kab mob DNA replication. Nws yog thawj hom DNA polymerase tshawb pom los ntawm Arthur Kornberg hauv xyoo 1956. Pol 1 yog cov polymerase ntau tshaj plaws hauv E. coli. Nws yog encoded los ntawm cov noob polA thiab muaj li ntawm 928 amino acids. Nws muaj peb lub npe. Tag nrho peb lub npe ua tiav cov haujlwm sib txawv. Ob tug thawj coj ua 5' rau 3' exonuclease kev ua thiab 3' rau 5' exonuclease kev ua. Vim tias qhov haujlwm no muaj los ntawm Pol 1, nws yog qhov nrov ua DNA kho enzyme es tsis yog DNA replicating enzyme. Lub npe thib peb muaj peev xwm ua kom muaj ntau yam polymerizations (5' rau 3') ua ntej tso tawm cov qauv DNA thiab txuas Okazaki fragments ua ke los ntawm kev sau cov DNA tshiab thiab tshem tawm RNA primers.
Daim duab 02: DNA Polymerase
Pol 1 cais los ntawm E Coli tau siv dav hauv cov ntawv thov molecular. Txawm li cas los xij, thaum tshawb pom Taq Polymerase, nws hloov E Coli Pol 1 hauv PCR thev naus laus zis. Taq polymerase yog hom thermostable DNA polymerase uas belongs rau Pol 1.
Dab tsi yog qhov zoo sib xws ntawm Klenow Fragment thiab DNA Polymerase 1?
- Klenow fragment yog ib feem loj ntawm DNA polymerase 1.
- Lawv muaj 5'to 3' DNA nyob ntawm DNA polymerase kev ua haujlwm thiab 3'to 5' exonuclease kev ua haujlwm uas kho cov ntaub ntawv pov thawj.
- Lawv siv rau ntau lub hom phiaj.
Qhov txawv ntawm Klenow Fragment thiab DNA Polymerase 1 yog dab tsi?
Qhov sib txawv tseem ceeb ntawm Klenow fragment thiab DNA polymerase 1 yog tias Klenow fragment tsis muaj 5′ txog 3′ exonuclease kev ua haujlwm thaum DNA polymerase 1 muaj 5′ rau 3′ exonuclease kev ua. Yog li ntawd, Klenow fragment muaj ob lub npe, thaum DNA polymerase 1 muaj tag nrho peb lub npe.
Hauv qab infographic qhia ntau cov ntsiab lus ntawm qhov sib txawv ntawm Klenow fragment thiab DNA polymerase 1.
Summary – Klenow Fragment vs DNA Polymerase 1
DNA polymerase 1 yog ib qho enzyme ntawm E. coli uas muaj peb lub npe raws li 5′ rau 3′ polymerization domain, 3′ rau 5′ exonuclease domain thiab 5′ rau 3′ exonuclease domain. Klenow fragment yog ib feem loj ntawm DNA polymerase. Nws tsis muaj ib qho ntawm DNA polymerase 1, uas yog 5' rau 3' exonuclease domain. Qhov sib txawv tseem ceeb ntawm Klenow fragment thiab DNA polymerase 1 yog tias Klenow fragments tsis muaj 5′ rau 3′ exonuclease domain thaum DNA polymerase 1 muaj 5′ rau 3′ exonuclease domain.
